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Journal of Peking University (Health Sciences) ›› 2024, Vol. 56 ›› Issue (1): 17-24. doi: 10.19723/j.issn.1671-167X.2024.01.004

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Effects of different polymers on biomimetic mineralization of small intestine submucosal scaffolds

Xiaoying CHEN1,Yi ZHANG2,Yuke LI1,Lin TANG1,*(),Yuhua LIU1,*()   

  1. 1. Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
    2. Department of General Dentistry Ⅱ, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2023-10-09 Online:2024-02-18 Published:2024-02-06
  • Contact: Lin TANG,Yuhua LIU E-mail:dent20@pku.edu.cn;liuyuhua@bjmu.edu.cn
  • Supported by:
    National Natural Science Foundation of China(81801027)

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Abstract:

Objective: To explore the effects of different polymers on in vitro biomimetic mineralization of small intestinal submucosa (SIS) scaffolds, and to evaluate the physicochemical properties and biocompatibility of the SIS scaffolds. Methods: The SIS scaffolds prepared by freeze-drying method were immersed in simulated body fluid (SBF), mineralized liquid containing polyacrylic acid (PAA) and mine-ralized liquid containing PAA and polyaspartic acid (PASP). After two weeks in the mineralized solution, the liquid was changed every other day. SBF@SIS, PAA@SIS, PAA/PASP@SIS scaffolds were obtained. The SIS scaffolds were used as control group to evaluate their physicochemical properties and biocompatibility. We observed the bulk morphology of the scaffolds in each group, analyzed the microscopic morphology by environment scanning electron microscopy and determined the porosity and pore size. We also analyzed the surface elements by energy dispersive X-ray spectroscopy (EDX), analyzed the structure of functional groups by Flourier transformed infrared spectroscopy (FTIR), detected the water absorption rate by using specific gravity method, and evaluated the compression strength by universal mechanical testing machine. The pro-cell proliferation effect of each group of scaffolds were evaluated by CCK-8 cell proliferation method. Results: Under scanning electron microscopy, the scaffolds of each group showed a three-dimensional porous structure with suitable pore size and porosity, and crystal was observed in all the mineralized scaffolds of each group, in which the crystal deposition of PAA/PASP@SIS scaffolds was more regular. At the same time, the collagen fibers could be seen to thicken. EDX analysis showed that the characteristic peaks of Ca and P were found in the three groups of mineralized scaffolds, and the highest peaks were found in the PAA/PASP@SIS scaffolds. FTIR analysis proved that all the three groups of mineralized scaffolds were able to combine hydroxyapatite with SIS. All the scaffolds had good hydrophilicity. The compressive strength of the mineralized scaffold in the three groups was higher than that in the control group, and the best compressive strength was found in PAA/PASP@SIS scaffold. The scaffolds of all the groups could effectively adsorb proteins, and PAA/PASP@SIS group had the best adsorption capacity. In the CCK-8 cell proliferation experiment, the PAA/PASP@SIS scaffold showed the best ability to promote cell proliferation with the largest number of living cells observed. Conclusion: Compared with other mineralized scaffolds, PAA/PASP@SIS scaffolds prepared by mineralized solution containing both PAA and PASP have better physicochemical properties and biocompatibility and have potential applications in bone tissue engineering.

Key words: Biopolymers, Biomimetic mineralization, Tissue engineering, Bone and bones, Small intestinal submucosa

CLC Number: 

  • R318

Figure 1

The macroscopic morphology of the scaffolds"

Figure 2

The microscopic morphology of the scaffolds by ESEM (A-D, ×300; E-H, ×1 000) ESEM, environment scanning electron microscopy."

Figure 3

The surface Mapping (A) and EDX spectra (B) of scaffolds CPS, counts per second; EDX, energy dispersive X-ray spectroscopy."

Figure 4

The physicochemical properties of scaffolds A, pore size; B, priority; C, water absorption; D, compressive modulus; E, protein absorption.* P < 0.05."

Figure 5

The mineralization properties and biocompatibility of scaffolds A, Flourier transformed infrared spectroscopy (FTIR); B, X-ray diffraction (XRD); C, dissolution; D, CCK-8 cell proliferation assay."

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