PPARγ mRNA在卵巢颗粒细胞的表达调节及与多囊卵巢综合征的相关性

摘要/Abstract

摘要： 目的：研究PPARγ mRNA在多囊卵巢综合征（polycystic ovary syndrome，PCOS）患者卵巢颗粒细胞的表达，以及不同浓度的睾酮、胰岛素及罗格列酮对卵巢颗粒细胞细胞核过氧化物酶体增殖激活受体γ（peroxisome proliferators-activated receptor γ，PPARγ）mRNA的表达调节。方法：分别提取5例PCOS患者和30例正常人卵巢颗粒细胞，对PCOS患者及5例正常人（对照组）颗粒细胞应用Real-time PCR方法检测PPARγ mRNA表达；剩余25例正常人卵巢颗粒细胞进行体外培养48 h后，分别用含不同浓度的睾酮、胰岛素及PPARγ激动剂（罗格列酮）的培养基培养颗粒细胞24 h后，应用Realtime PCR方法检测PPARγ mRNA的表达。结果：PCOS患者卵巢颗粒细胞PPARγ mRNA的表达明显低于对照组（P＜0.05）。睾酮浓度为1 nmol/L时，PPARγ mRNA的表达明显减少（P＜0.05）；睾酮浓度为10 nmol/L时，PPARγ mRNA的表达升高，与对照组差异无统计学意义（P＞0.05）。胰岛素浓度为10 nmol/L时，PPARγ mRNA的表达较对照组明显增加（P＜0.05）；当胰岛素浓度为100 nmol/L时，PPARγ mRNA的表达与胰岛素浓度为10 nmol/L时差异无统计学意义（P＞0.05）。罗格列酮浓度为1 nmol/L时，PPARγ mRNA的表达较对照组明显增加（P＜0.05）；当罗格列酮浓度为10 nmol/L时，PPARγ mRNA的表达较罗格列酮浓度为1 nmol/L时明显增加（P＜0.05）。结论：卵巢颗粒细胞PPARγ mRNA的表达与睾酮的浓度有关，胰岛素诱导颗粒细胞PPARγ mRNA的表达，颗粒细胞PPARγ mRNA的表达受PPARγ的正向调节，PCOS患者卵巢颗粒细胞PPARγ mRNA的表达异常可能与PCOS的发生有关。

关键词: 多囊卵巢综合征, PPARγ, 颗粒细胞

Abstract: To study the expression of PPARγ mRNA in granulosa cells of patients with polycystic ovary syndrome (PCOS) and the impact of testosterone, insulin and PPARγ agonist rosiglitazone on granulosa cells (GCs).Methods: The expression of PPARγ mRNA in GCs of patients with PCOS and normal controls were analyzed by Real-time PCR. We assessed the level of PPARγ mRNA in GCs from normal controls after treatment with testosterone, insulin, and rosiglitazone.Results: The expression of PPARγ mRNA was lower in the GCs of PCOS than that of the controls (P<0.05). When testosterone concentration was 1 nmol/L, the expression of PPARγ mRNA was lower in the GCs as compared with the blank control (P<0.05). When testosterone concentration was 10 nmol/L, PPARγ mRNA increased in the GCs as compared with the blank control, which was of no significance (P>0.05). When insulin concentration was 10 nmol/L, the expression of PPARγ mRNA was higher in the GCs as compared with the blank control (P<0.05). When insulin concentration was 100 nmol/L, the expression of PPARγ mRNA increased, but the difference was not statistically significant (P>0.05). When rosiglitazone concentration was 1 nmol/L, the expression of PPARγ mRNA in ovarian GCs significantly increased, as compared with the blank control (P<0.05). When rosiglitazone concentration was at 10 nmol/L, the PPARγ mRNA expression significantly increased, as compared with the concentration at 1 nmol/L (P<0.05).Conclusion: PPARγ mRNA expression is down-regulated by testosterone, and up-regulated by insulin and rosiglitazone with different dosages. Decreased PPARγ mRNA in the GCs of PCOS is related to the clinical characteristics of PCOS.

Key words: Polycystic ovary syndrome, PPAR gamma, Granulosa cells

胡卫红，陈琳，同军，赵春艳，毛莎，乔杰. PPARγ mRNA在卵巢颗粒细胞的表达调节及与多囊卵巢综合征的相关性[J]. 北京大学学报（医学版）, 2013, 45(6): 859-863.

HU Wei-hong, CHEN Lin, TONG Jun, ZHAO Chun-yan, MAO Sha, QIAO Jie. Expression of PPARγ mRNA in granulosa cells and its correlation with clinical characteristics of polycystic ovary syndrome[J]. Journal of Peking University(Health Sciences), 2013, 45(6): 859-863.

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