激活辣椒素受体是调控下颌下腺分泌的新途径

北京大学学报（医学版） ›› 2015, Vol. 47 ›› Issue (1): 8-12.

激活辣椒素受体是调控下颌下腺分泌的新途径

丛馨1, 2，张艳1, 2，俞光岩3，吴立玲1, 2△

（1. 北京大学口腔医学院·口腔医院唾液腺疾病研究中心，北京100081；2. 北京大学医学部基础医学院生理学与病理生理学系，北京100191；3. 北京大学口腔医学院·口腔医院口腔颌面外科，北京100081）

出版日期:2015-02-18 发布日期:2015-02-18

Activation of transient receptor potential vanilloid subtype 1 serves as a novel pathway to modulate secretion in submandibular gland

CONG Xin1, 2 , ZHANG Yan1, 2, YU Guang-yan2, WU Li-ling1, 2△

(1. Center for Salivary Gland Diseases, Peking University School and Hospital of Stomatology, Beijing 100081, China; 2. Department of Physiology and Pathophysiology, Peking University School of Basic Medical Sciences, Beijing 100191, China; 3. Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing 100081, China)

Online:2015-02-18 Published:2015-02-18

摘要/Abstract

关键词: 辣椒素受体亚型1, 颌下腺, 水通道蛋白质类

Abstract: Our work focused on the studies on the expression and function of transient receptor potential vanilloid subtype 1 (TRPV1) in the submandibular gland. By using reverse transcription-polymerase chain reaction (RT-PCR), Western blotting, and immunofluorescence, our data demonstrated the expression and distribution characteristics of TRPV1 in rabbit and human submandibular glands, as well as rat submandibular gland cell line SMG-C6. Furthermore, the possible intracellular signal molecules involved in the TRPV1-modulated saliva secretion were explored. Activation of TRPV1 increased the intracellular Ca²⁺ concentration, upregulated the expression of aquaporin 5 (AQP5), the main transporter that mediate water secretion through transcellular pathway, and led to AQP5 redistribution. Extracellular signal-regulated kinase 1/2 (ERK1/2) was involved in the TRPV1-regulated AQP5 content. Besides, TRPV1 activation also modulated the expression, distribution, and function of tight junction protein, and increased paracellular permeability. ERK1/2 and myosin light chain 2 (MLC2) were responsible for the regulation of TRPV1on tight junction properties. Taken together, our work suggested that TRPV1 was a potential target to promote saliva secretion, and activation of TRPV1 might provide a new and safe therapeutic strategy to ameliorate submandibular gland hypofunction.

Key words: Vanilloid receptor subtype 1, Submandibular gland, Aquaporins

丛馨, 张艳, 俞光岩, 吴立玲. 激活辣椒素受体是调控下颌下腺分泌的新途径[J]. 北京大学学报（医学版）, 2015, 47(1): 8-12.

CONG Xin, ZHANG Yan, YU Guang-Yan, WU Li-Ling. Activation of transient receptor potential vanilloid subtype 1 serves as a novel pathway to modulate secretion in submandibular gland[J]. Journal of Peking University(Health Sciences), 2015, 47(1): 8-12.

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