关键词: 心脏肥大, 纤维化, 异丙肾上腺素, 模型, 动物, 小鼠

Abstract: Objective： To investigate the condition of isoprenaline (ISO)induced cardiac hypertrophy in the FVB/N mouse. Methods: ISO ［30 mg/(kg·d)］ was administered either by daily subcutaneous injection, or by continuous infusion via an implanted osmotic minipump. The mice in each mode of administration were randomly divided into two groups. For subcutaneous injection: the mice received ISO or saline through daily subcutaneous injection for 2 weeks. The mice for minipump: the mice received continuous infusion of ISO via an implanted osmotic minipump for 2 weeks, or received sham operation as the control to mimipump. The ratio of heart weight to tibia length (HW/TI), the diastolic left ventricular posterior wall thickness (dLVPW) were used to indicate cardiac hypertrophy. Interstitial fibrosis was examined with picrosirius red staining. Results: ISO ［30 mg/(kg·d)］ administered by daily subcutaneous injection did not lead to cardiac hypertrophy or fibrosis in the FVB/N mice, and 50% of the mice died before the end point. The mice receiving ISO via minipumps showed significant increase in HW/TI ［(10.60±0.40 ) mg/mm vs. (7.93±0.19) mg/mm，P<0.001］ and dLVPW ［（0.87±0.03）mm vs. （0.68±0.06）mm，P=0.0116］. ISO administered via minipumps did not induce cardiac fibrosis. All the mice in this group survived to the end point. Conclusion: ISO ［30 mg/(kg·d)］ administered by continuous infusion via a minipump for 2 weeks can lead to significant cardiac hypertrophy.

Key words: Cardiomegaly, Fibrosis, IsoprenalineIsoprenaline, IsoprenalineModels, animal, IsoprenalineMice