寡发酵链球菌在大鼠口腔内定植状态的观察

doi:10.3969/j.issn.1671-167X.2016.02.025

摘要/Abstract

摘要：

目的：对寡发酵链球菌在大鼠口腔内高糖环境下的定植能力进行初步探索。方法：21天龄SPF-SD大鼠48只，24~27天喂以氨苄青霉素水溶液（内源性细菌抑制），28天时将大鼠分为4组，每组12只，高糖持续饲喂，28~30天连续3天接种菌液，组1（SM组）接种变形链球菌菌液，组2（SO组）接种寡发酵链球菌菌液，组3（SO+SM混合接种组）接种变形链球菌和寡发酵链球菌混合菌液，组4为阴性对照组，不接种任何菌液。细菌接种次日和第10天，用无菌棉签擦取大鼠双侧下颌磨牙（6颗）牙合面、颊、舌面的菌斑，PBS系列稀释，SM组接种于MSB平皿和BHIS血平皿，SO组接种于MSAE平皿，SO+SM混合接种组接种于MSB平皿、MSAE平皿和BHIS血平皿，阴性对照组接种于MSB平皿和MSAE平皿。从MSB平皿筛选、计数变形链球菌，从MSAE平皿筛选寡发酵链球菌疑似菌落并16S rDNA测序鉴定寡发酵链球菌。结果：SO组接种寡发酵链球菌次日，寡发酵链球菌检出率为33.3% (4/12)；SO+SM混合接种组接种次日寡发酵链球菌检出率为0，而变形链球菌检出率为100.00%，变形链球菌占总菌的比例为14.70%±4.53%；SM组在接种次日的变形链球菌检出率也为100.00%，其占总菌的比例为12.42%±4.27%，与SO+SM混合接种组相比差异无统计学意义。SO组接种寡发酵链球菌第10天，寡发酵链球菌检出率为0；SO+SM混合接种组接种第10天，寡发酵链球菌检出率亦为0，而变形链球菌检出率为100.00%，变形链球菌占总菌的比例为15.78%±5.10%；SM组在接种第10天的变形链球菌检出率也为100%，其占总菌的比例为17.08%±5.75%，与SO+SM混合接种组相比差异无统计学意义。结论：本实验条件下，在大鼠口腔内高糖环境下，寡发酵链球菌在大鼠口腔内呈一过性显现，不能定植。

关键词: 寡发酵链球菌, 链球菌, 变异, 大鼠, Sprague-Dawley

Abstract:

Objective：To study the colonization ability of Streptococcus oligofermentans (S. oligofermentan) in the condition of high sucrose in oral cavity of rats. Methods: In this study, 48 SPF-SD rats aged 21 days were selected. From 24th to 27th days, the rats were fed with water of antibiotic and fed with high glucose diet continuously. On the 28th day, the rats were divided into four groups randomly, 12 rats per group. From the 28th day to 30th day, the first group (SM group) was inoculated with S. mutans, the second group (SO group) with S. oligofermentan, the third group (SO+SM group) with mixture of S. mutans and S. oligofermentan, the control group not with any bacteria. On the next day and the 10th day after inoculation of bacteria, the samples of dental plaque of the rats were acquired by scrubbing occlusal, buccal and lingual surfaces of bilateral mandibular molars with sterile swabs. The samples of SM group were inoculated on MSB and BHIS, of SO group on MSAE, of SO+SM group on MSB, MSAE and BHIS,of the control group on MSB and MSAE. S. mutans were screened and calculated on MSB, the suspected colonies of S. oligofermentan were screened and identified by the analysis of 16S rDNA. Results:On the next day, the detection rate of S. oligofermentan was 33.3% (4/12) in the group of SO; in the group of SO+SM, the detection rate of S. oligofermentan was 0, the detection rate of S. mutans 100.00%, and the proportion of S. mutans 14.70%±4.53%; in the group of SM, the detection rate of S. mutans was 100.00%, the proportion of S. mutans 12.42%±4.27%. On the 10th day, in the group of SO, the detection rate of S. oligofermentan was 0; in the group of SO+SM, the detection rate of S. oligofermentan was 0, the detection rate of S. mutans 100.00%, and the proportion of S. mutans 15.78%±5.10%; in the group of SM, the detection rate of S. mutans was 100.00%,and the proportion of S. mutans 17.08%±5.75%. Conclusion: In the condition of the experiment where high glucose was maintained in the oral cavity in rats, S. oligofermentan appeared transiently and couldn’t colonize in the rats.

Key words: Streptococcus oligofermentans, Streptococcus mutans, Rats, Sprague-Dawley

中图分类号:

R378.12

张杰，宋磊，段登辉，岳林. 寡发酵链球菌在大鼠口腔内定植状态的观察[J]. 北京大学学报(医学版), 2016, 48(2): 316-319.

ZHANG Jie, SONG Lei, DUAN Deng-hui, YUE Lin. Observation of oral Streptococcus oligofermentans colonization in rats[J]. Journal of Peking University(Health Sciences), 2016, 48(2): 316-319.

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