交联聚乙烯醇栓塞微球的制备与体外性质

摘要/Abstract

摘要： 目的：研究交联聚乙烯醇栓塞微球（polyvinyl alcohol microspheres，PVA-Ms）的制备方法及其理化性质。方法:采用乳化化学交联法制备PVA-Ms，以红外光谱法考察微球交联产物的结构，以光学显微镜观察微球的形态、测定粒径大小，考察微球的吸水率和溶胀率，以物性测定仪测量微球的弹性，采用创新设计的装置模拟栓塞推注微球的过程并实时测定推注压力。结果: 红外光谱确证了微球为聚乙烯醇与甲醛的交联产物。制备的PVA-Ms圆整，冻干微球的粒径范围为80~1 800 μm，平均粒径574.2 μm；湿微球的粒径范围为100~1 900 μm，平均粒径602.2 μm。冻干微球在生理盐水中20 min内达到吸水平衡，平均吸水率为175%，平均溶胀率为48.6%。微球具有良好的弹性，可顺利经导管推注，大粒径的微球通过导管的压力较大。结论:PVA-Ms的理化性质可以满足栓塞治疗的要求，本研究为系统评价栓塞微球的体外性质提供了方法。

关键词: 栓塞, 治疗性, 微球体, 聚乙烯乙醇, 交联试剂

Abstract: Objective：To develop and study the properties of crosslinked polyvinyl alcohol microspheres (PVA-Ms) for embolization. Methods: The PVA-Ms were produced by emulsion chemical crosslinking method. Fourier transform infrared spectroscopy (FT-IR) was used to investigate the special functional groups of PVA-Ms; the morphology and particle size of PVA-Ms were determined by optical microscope; the ratio of water absorption and the swelling ratio were also investigated; the compressibility was examined by texture analyzer. A new device was designed to measure the pressure of PVA-Ms during their delivery through catheter for embolization. Results: The crosslinking reaction of PVA and formaldehyde was proved by FT-IR. The PVA-Ms were round with smooth surface. The average diameter of lyophilized PVA-Ms was 574.2 μm with a range of 80~1 800 μm and of wet PVA-Ms was 602.2 μm with a range of 100~1 900 μm. The average ratio of water absorption was 175% and the swelling ratio was 48.6%. The PVA-Ms were mechanically stable with appropriate elasticity and delivered through the catheter without any difficulty, and the pressure was higher for larger size of microspheres to be delivered. Conclusion: PVA-Ms prepared in this study was supposed to be suitable for clinical embolization according to the physicochemical properties. The study provides a series of methods to evaluate the properties of microspheres systemically for embolization in vitro.

Key words: Embolization, therapeutic, Microspheres, Polyvinyl alcohol, Cross-linking reagents

杨棽, 孟文静, 卢晓静, 吴雅楠, 仁增顿珠, 范田园. 交联聚乙烯醇栓塞微球的制备与体外性质[J]. 北京大学学报（医学版）, 2014, 46(5): 733-738.

YANG Chen, MENG Wen-Jing, LU Xiao-Jing, WU Ya-Nan, REN Zeng-顿Zhu, FAN Tian-Yuan. Preparation and in vitro evaluation of crosslinked polyvinyl alcohol microspheres for embolization[J]. Journal of Peking University(Health Sciences), 2014, 46(5): 733-738.

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