交联聚乙烯醇栓塞微球的制备与体外性质

摘要/Abstract

摘要： 目的：研究交联聚乙烯醇栓塞微球（polyvinyl alcohol microspheres，PVA-Ms）的制备方法及其理化性质。方法:采用乳化化学交联法制备PVA-Ms，以红外光谱法考察微球交联产物的结构，以光学显微镜观察微球的形态、测定粒径大小，考察微球的吸水率和溶胀率，以物性测定仪测量微球的弹性，采用创新设计的装置模拟栓塞推注微球的过程并实时测定推注压力。结果: 红外光谱确证了微球为聚乙烯醇与甲醛的交联产物。制备的PVA-Ms圆整，冻干微球的粒径范围为80~1 800 μm，平均粒径574.2 μm；湿微球的粒径范围为100~1 900 μm，平均粒径602.2 μm。冻干微球在生理盐水中20 min内达到吸水平衡，平均吸水率为175%，平均溶胀率为48.6%。微球具有良好的弹性，可顺利经导管推注，大粒径的微球通过导管的压力较大。结论:PVA-Ms的理化性质可以满足栓塞治疗的要求，本研究为系统评价栓塞微球的体外性质提供了方法。

关键词: 栓塞, 治疗性, 微球体, 聚乙烯乙醇, 交联试剂

Abstract: Objective：To develop and study the properties of crosslinked polyvinyl alcohol microspheres (PVA-Ms) for embolization. Methods: The PVA-Ms were produced by emulsion chemical crosslinking method. Fourier transform infrared spectroscopy (FT-IR) was used to investigate the special functional groups of PVA-Ms; the morphology and particle size of PVA-Ms were determined by optical microscope; the ratio of water absorption and the swelling ratio were also investigated; the compressibility was examined by texture analyzer. A new device was designed to measure the pressure of PVA-Ms during their delivery through catheter for embolization. Results: The crosslinking reaction of PVA and formaldehyde was proved by FT-IR. The PVA-Ms were round with smooth surface. The average diameter of lyophilized PVA-Ms was 574.2 μm with a range of 80~1 800 μm and of wet PVA-Ms was 602.2 μm with a range of 100~1 900 μm. The average ratio of water absorption was 175% and the swelling ratio was 48.6%. The PVA-Ms were mechanically stable with appropriate elasticity and delivered through the catheter without any difficulty, and the pressure was higher for larger size of microspheres to be delivered. Conclusion: PVA-Ms prepared in this study was supposed to be suitable for clinical embolization according to the physicochemical properties. The study provides a series of methods to evaluate the properties of microspheres systemically for embolization in vitro.

Key words: Embolization, therapeutic, Microspheres, Polyvinyl alcohol, Cross-linking reagents

杨棽, 孟文静, 卢晓静, 吴雅楠, 仁增顿珠, 范田园. 交联聚乙烯醇栓塞微球的制备与体外性质[J]. 北京大学学报（医学版）, 2014, 46(5): 733-738.

YANG Chen, MENG Wen-Jing, LU Xiao-Jing, WU Ya-Nan, REN Zeng-顿Zhu, FAN Tian-Yuan. Preparation and in vitro evaluation of crosslinked polyvinyl alcohol microspheres for embolization[J]. Journal of Peking University(Health Sciences), 2014, 46(5): 733-738.

参考文献

相关文章 15

[1]	汤晓菲,李永红,丁秋玲,孙卓,张阳,王育梅,田美伊,刘坚. 类风湿关节炎患者下肢深静脉血栓发病率及危险因素[J]. 北京大学学报（医学版）, 2024, 56(2): 279-283.
[2]	彭清,刘佳君,刘焱,尚华,唐果,韩雅欣,龙丽. Padua预测评分和血清白蛋白水平在评估风湿病住院患者静脉血栓栓塞中的应用[J]. 北京大学学报（医学版）, 2023, 55(4): 625-630.
[3]	韩金涛,张宇翔,贾子昌,姜除寒,刘恋,栾景源,梁飞,赵彦清. Neuroform Atlas支架辅助弹簧圈栓塞未破裂性颅内宽颈动脉瘤[J]. 北京大学学报（医学版）, 2023, 55(1): 139-143.
[4]	袁昌巍,王盈进,张书杰,沈胜利,段鸿洲. 显微外科手术与血管内栓塞治疗硬脊膜动静脉瘘临床疗效比较的meta分析[J]. 北京大学学报（医学版）, 2022, 54(2): 304-314.
[5]	朱冯赟智,邢晓燕,汤晓菲,李依敏,邵苗,张学武,李玉慧,孙晓麟,何菁. 肌炎合并血栓栓塞患者的临床及免疫学特征[J]. 北京大学学报（医学版）, 2020, 52(6): 995-1000.
[6]	高健,胡立宝,陈尘,郅新,徐涛. 经皮肾镜去石术后出血的介入治疗[J]. 北京大学学报（医学版）, 2020, 52(4): 667-671.
[7]	赵海燕,樊东升,韩金涛. 重度颈内动脉狭窄伴未破裂动脉瘤的治疗策略[J]. 北京大学学报（医学版）, 2019, 51(5): 829-834.
[8]	张志刚,刘新民. 急性肾梗死的临床特征:单中心52例临床分析[J]. 北京大学学报（医学版）, 2019, 51(5): 863-869.
[9]	张玮,张培训. 老年髋部骨折患者围手术期血栓预防时限分析[J]. 北京大学学报（医学版）, 2019, 51(3): 501-504.
[10]	顾婕昱,陆翠,石慧,杨程德. 14例恶性抗磷脂综合征病例报道及临床分析[J]. 北京大学学报（医学版）, 2018, 50(6): 1033-1038.
[11]	郭李盈,刘晓昕,李子圆,覃小雅,范则杨,李真真,关海涛,宋莉,邹英华,范田园. 空白及载阿霉素的聚丙烯酸栓塞微球的制备与评价[J]. 北京大学学报（医学版）, 2018, 50(6): 1070-1077.
[12]	赵灿，胡京敏，郭丹杰. 血浆D二聚体临界值联合Wells量表对可疑肺栓塞的除外价值[J]. 北京大学学报(医学版), 2018, 50(5): 828-832.
[13]	于路平，赵卫红，刘士军，李清，徐涛. 先天性肾动静脉瘘伴肾动脉多支畸形病例分析[J]. 北京大学学报(医学版), 2018, 50(4): 722-728.
[14]	鲁景元，顾建平，徐文健，楼文胜，施万印，汪涛，邵泽锋. 切口妊娠介入治疗的临床应用及预后分析[J]. 北京大学学报(医学版), 2016, 48(6): 1012-1018.
[15]	李箐，于欣，周钢，毋育伟，胡洪成，王彤，唐志辉. 壳聚糖微球/小牛松质骨支架复合缓释体系的制备及体外生物活性评价[J]. 北京大学学报(医学版), 2016, 48(6): 1043-1048.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed