北京大学学报(医学版) ›› 2015, Vol. 47 ›› Issue (1): 42-46.

• 论著 • 上一篇    下一篇

两种钙黏蛋白在小鼠牙齿发育期的时间和空间表达

赵双云1,2,刘明2,赵玉鸣1,林庆杰2,3,WANG Xiuping2△,葛立宏1△   

  1. (1. 北京大学口腔医学院·口腔医院儿科,北京100081;2. Department of Developmental Biology, Harvard School of Dental Medicine, Boston 02115, USA; 3. 山东大学口腔医学院种植科,济南250012)
  • 出版日期:2015-02-18 发布日期:2015-02-18

Spatiotemporal expression pattern of E-cadherin and P-cadherin during mouse tooth development

ZHAO Shuang-yun1,2, LIU Ming2, ZHAO Yu-ming1, LIN Qing-jie2,3, WANG Xiu-ping2△, GE Li-hong1△   

  1. (1. Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China; 2. Department of Developmental Biology, Harvard School of Dental Medicine, Boston 02115, USA; 3. Department of Implantation, School of Stomatology, Shandong University, Jinan 250012, China)
  • Online:2015-02-18 Published:2015-02-18

摘要: 目的:观察E-钙黏蛋白及P-钙黏蛋白在牙齿胚胎发育早期的时间和空间分布。方法:取胚胎第12.5天至出生后第3.5天的CD1型小鼠下颌骨标本,免疫荧光二步法及共聚焦荧光显微镜检测E-钙黏蛋白及P-钙黏蛋白在下颌第一磨牙牙胚发育不同时期的表达情况,通过HE染色显示组织形态。结果:两种钙黏蛋白在牙胚发育过程中广泛表达于上皮组织中,前成釉细胞在极化过程中E-钙黏蛋白表达增强、P-钙黏蛋白表达减弱,P-钙黏蛋白在牙胚发育初期即出现于牙胚上皮表层,并在分泌期成釉细胞中表达。结论:钙黏蛋白在牙胚早期发育过程中存在空间、时间上的表达差异,提示两种钙黏蛋白在牙冠形成期间行使不同的功能,推测P-钙黏蛋白参与了釉质的分泌与矿化过程。

关键词: 钙黏着糖蛋白类, 牙胚, 荧光抗体技术

Abstract: Objective: To investigate the expression patterns of E-cadherin and P-cadherin in murinetooth germs at early developmental stages. Methods: Mandible samples of CD1 mice from embryonic day 12.5 to postnatal day 3.5 were collected. The expressions of E-cadherin and P-cadherin in murine mandibular first molar germs were detected by immunofluorescence and observed under confocal fluorescence microscope. HE staining was performed for tissue morphology. Results: Both E-cadherin and P-cadherin were widely expressed in the epithelial tissues through early developmental stages. The E-cadherin expression was increased in polarizing pre-ameloblasts, whereas the P-cadherin expression declined. The expression of the P-cadherin could be detected in epithelial tissues before bud stage, and expressed in mature ameloblasts at secretory stage. Conclusion: The E-cadherin and P-cadherin expressed in different spatiotemporal expression patterns, indicating their individual functions during tooth development. P-cadherin might function in the secretion and mineralization of enamel.

Key words: Cadherins, Tooth germ, Fluorescent antibody technique

[1] 田靖,秦满,陈洁,夏斌. 失活剂烧伤致乳磨牙早失及恒牙胚丧失2例[J]. 北京大学学报(医学版), 2022, 54(2): 381-385.
[2] 胡浩,陈京文,许克新,王栋,王应,王功伟,张石英,王晓峰. CMTM8与E-cadherin在原发性和转移性肾透明细胞癌中的表达[J]. 北京大学学报(医学版), 2013, 45(4): 537-.
[3] 张燕, 陈剑, 刘海静, 陆敏. 石蜡切片免疫荧光法在肾病穿刺组织中的应用[J]. 北京大学学报(医学版), 2011, 43(6): 900-902.
[4] 刘畅, 曲贞, 杨瑞, 赵娟, 贾晓玉, 郑欣, 刘章锁, 崔昭, 赵明辉. 抗肾小球基底膜病患者血清自身抗体的异质性及其临床病例的相关性分析[J]. 北京大学学报(医学版), 2009, 41(6): 625-629.
[5] 吕平, 高学军, 高岩, 贾弘禔. 釉蛋白在大鼠牙胚釉质发育中的表达特征[J]. 北京大学学报(医学版), 2007, 39(1): 37-40.
[6] 周彦秋, 林久祥. RT-PCR法研究sonic hedgehog基因及其受体patched在小鼠牙胚钟状晚期的表达[J]. 北京大学学报(医学版), 2002, 34(2): 137-139.
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