北京大学学报(医学版) ›› 2017, Vol. 49 ›› Issue (3): 376-382. doi: 10.3969/j.issn.1671-167X.2017.03.002

• 论著 • 上一篇    下一篇

体外翻转肠囊法研究纳米二氧化钛对幼年大鼠小肠葡萄糖吸收的影响

张永亮,陈章健,陈实,卓琳,贾光,王云△   

  1. (北京大学公共卫生学院劳动卫生与环境卫生学系, 北京100191)
  • 出版日期:2017-06-18 发布日期:2017-06-18
  • 通讯作者: 王云 E-mail: wangyun@bjmu.edu.cn
  • 基金资助:
    国家自然科学基金青年基金项目(31400863)和北京市自然科学基金资助项目(7172116)资助

Effect of nano-TiO2 on intestinal glucose absorption in young rat on the everted gut sac model

ZHANG Yong-liang, CHEN Zhang-jian, CHEN Shi, ZHUO lin, JIA Guang, WANG Yun△   

  1. (Department of Occupational and Environmental Health Science, Peking University School of Public Health, Beijing 100191, China)
  • Online:2017-06-18 Published:2017-06-18
  • Contact: WANG Yun E-mail: wangyun@bjmu.edu.cn
  • Supported by:
    Supported by the National Natural Science Foundation of China (31400863) and the Beijing Natural Science Foundation (7172116)

摘要:  目的:探究纳米二氧化钛(TiO2)对幼年大鼠小肠葡萄糖吸收功能的影响及其尺寸效应。方法:取63只幼年(4周龄)雄性Sprague-Dawley(SD)大鼠的小肠,建立63个体外翻转肠囊模型,研究葡萄糖浓度分别为10、25、50、100、200、400、800 mmol/L时,暴露于0、50 mg/L纳米TiO2(24 nm)2 h对小肠葡萄糖吸收功能的影响;并在此基础上,继续利用体外翻转肠囊模型,在400 mmol/L葡萄糖浓度下,研究暴露于0、10、50、200 mg/L纳米TiO2(24 nm)和微米TiO2(120 nm)2 h对小肠葡萄糖吸收功能的影响。每组3个肠囊。结果:幼年大鼠小肠的葡萄糖累积吸收量随葡萄糖浓度和染毒时间的增加而增加,仅在400 mmol/L葡萄糖浓度下,观察到50 mg/L纳米TiO2染毒组的葡萄糖累积吸收量和吸收速率在30 min(t累积吸收量=3.254, P<0.05;t吸收速率=3.958,P<0.05)、90 min(t累积吸收量=3.323, P<0.05;t吸收速率=3.063,P<0.05)、120 min(t累积吸收量=2.834, P<0.05;t吸收速率=3.002,P<0.05)时显著低于对照组。第二步研究中,与对照组相比,不同剂量纳米TiO2或微米TiO2染毒对幼年大鼠小肠葡萄糖的累积吸收量及乳酸累积生成量无明显影响。在相同染毒时间及染毒剂量下,未观察到纳米和微米TiO2染毒组组间葡萄糖累积吸收量及乳酸累积生成量的差异有统计学意义。 结论:纳米TiO2短期暴露可能对幼年大鼠小肠葡萄糖吸收具有一定的抑制作用,且与微米TiO2的差异不明显。

关键词: 二氧化钛, 葡萄糖吸收, 翻转肠囊

Abstract: Objective: To study the effect of titanium dioxide (TiO2) nanoparticles on intestinal glucose absorption in young rats and its size effect. Methods: In the study, 63 small intestine segments were isolated from 63 Sprague-Dawley rats (SD rats, 4-week-old) to prepare the everted gut sac model. In the first part of our work, the everted sacs were exposed to 0, 50 mg/L TiO2 nanoparticles (24 nm) for 2 h with the presence of a series of glucose concentrations (10, 25, 50, 100, 200, 400, and 800 mmol/L), and the glucose absorbing function of the everted sacs were assessed in the process. On the basis of the work, utilizing the same method, further study was carried out to compare the effects of TiO2 nanoparticles (24 nm) and fine-particles (120 nm) on intestinal glucose absorbing function with the presence of 400 mmol/L glucose and 0, 10, 50, 200 mg/L TiO2. 3 intestine segments were used in each group. Results: The cumulative glucose absorption increased with time extension and increased glucose concentration. In the first part of our work, with the presence of 400 mmol/L glucose, the group treated with 50 mg/L TiO2 nanoparticles showed significantly lower cumulative glucose absorption and glucose absorbing rate than the control group at the exposure time of  30 min (tcumulative absorption=3.254, P<0.05; tglucose absorbing rate=3.958, P<0.05), 90 min (tcumulative absorption=3.323, P<0.05; tglucose absorbing rate=3.063, P<0.05) and 120 min (tcumulative absorption=2.834, P<0.05; tglucose absorbing rate=3.002, P<0.05). At other glucose concentrations, statistically significant differences in cumulative glucose absorption or glucose absorbing rates were not found between the TiO2 nanoparticle exposed group and the control group. In the second part of our work, when compared with the control group, no significant downregulations in cumulative glucose absorption or glucose absorbing rates were observed in both TiO2 nano-particle treated group and TiO2 fine particle treated group. Differences between the TiO2 nanoparticle treated group and the TiO2 fine particle treated group were not statistically significant. Conclusion: Short-term exposure to TiO2 nanoparticles may downregulate the intestinal glucose absorbing function in young rats, and the difference with TiO2 fine particlesis is not obvious.

Key words: Titanium dioxide, Glucose absorption, Everted gut sac

中图分类号: 

  • R318.08
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