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Journal of Peking University(Health Sciences) ›› 2020, Vol. 52 ›› Issue (1): 51-57. doi: 10.19723/j.issn.1671-167X.2020.01.008

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Characteristics of orofacial operant test for orofacial pain sensitivity caused by occlusal interference in rats

Shan-shan BAI1,2,Si-yi MO1,2,Xiao-xiang XU1,2,Yun LIU1,2,Qiu-fei XIE1,2,△(),Ye CAO1,2,△()   

  1. 1. Center for Oral and Jaw Functional Diagnosis, Peking University School and Hospital of Stomatology & Department of Prosthodontics, Beijing 100081, China
    2. Center for Oral and Jaw Functional Diagnosis, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2019-10-10 Online:2020-02-18 Published:2020-02-20
  • Contact: Qiu-fei XIE,Ye CAO E-mail:xieqiuf@163.com;ye.cao@bjmu.edu.cn
  • Supported by:
    Supported by the Beijing Natural Science Foundation(7192231)

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Abstract:

Objective: To compare the orofacial pain sensitivity with operant test and mechanical hyperalgesia with von Frey filaments of two orofacial pain models (EOI: experimental occlusal interference; pIONX: partial infraorbital nerve transection). To investigate the operant and evoked characteristics of EOI-rats. Methods: The orofacial operant behaviors were tested by Ugo Basile Orofacial Stimulation Test System. The mechanical thresholds of vibrissal pads were tested by von Frey filaments. Male Sprague-Dawley rats were randomly divided into eight groups: von Frey group: sham-EOI, EOI, sham-pIONX, pIONX (sham: sham-operated group); operant test group: sham-EOI, EOI, sham-pIONX, pIONX (sham: sham-operated group). The mechanical thresholds and orofacial operant behaviors were tested on pre-operation and post-operation days l, 3, 7, 10, 14 and 21. Results: In pIONX of von Frey group, the mechanical withdrawal threshold decreased from days 1 to 21 (P<0.05), peaking from days 7 to 10, and lasted until the end of the experiment. There was no significant difference between the bilateral sides. In pIONX of operant test group, the total contact time decreased from days 10 to 21 (P<0.05), peaking from days 10 to 14, and lasted until the end of the experiment. In EOI of von Frey group, the mechanical withdrawal threshold decreased from days 3 to 21 (P<0.05), peaking on day 7, and lasted until the end of the experiment. There was no significant difference between the bilateral sides. In EOI of operant test group, the total contact time decreased from days 1 to 21 (P<0.05), peaking from days 7 to 10, and lasting until the end of experiment. Conclusion: Orofacial operant test is a stable method to evaluate orofacial pain behaviors, which could discriminate the feature of neuropathic and EOI orofacial pain. In these two animal models, both of the operant behaviors and the mechanical hyperalgesia exhibited different time courses. Orofacial operant test provides a novel method for evaluating the orofacial pain sensitivity and studying the orofacial pain mechanism thoroughly.

Key words: Animal behavior, Facial pain, Hyperalgesia, Animal disease models, Rats

CLC Number: 

  • R782

Figure 1

Ugo Basile Orofacial Stimulation Test System A, diagram of the orofacial operant test system; B, the blank mask and three mechanical modules with different number of wires."

Figure 2

Weight change of the intermittent fasting rats and free-diet rats (n=6) * P<0.05."

Figure 3

Mechanical withdrawal threshold of the sham-pIONX group and pIONX group * P<0.05, significant difference between the sham-pIONX ipsilateral and pIONX ipsilateral; # P<0.05, significant difference between the sham-pIONX contralateral and pIONX contralateral."

Figure 4

The operant behavior of the sham-pIONX group and pIONX group A, total contact time of orofacial operant tests between two groups; B, total contact numbers of the two groups; C, a sample trace shows the automatic recordings of operant behavior in a duration of 10 min of a pre-operation pIONX rat; D, a sample trace of a post-operation pIONX rat. * P<0.05, significant difference between the two groups; # P<0.05, significant difference between the baseline and days after operation."

Figure 5

Mechanical withdrawal threshold of the sham-EOI group and EOI group * P<0.05, significant difference in the ipsilateral sides between the sham-EOI and EOI groups; # P<0.05, significant difference in the contralateral sides between the sham-EOI and EOI groups."

Figure 6

The operant behavior of the sham-EOI group and EOI group A, total contact time of orofacial operant tests between two groups; B, total contact numbers of the two groups; C, a sample trace shows the automatic recordings of operant behavior in a duration of 10 min of a pre-operation EOI-rat; D, a sample trace of a post-operation EOI-rat. * P<0.05, significant difference between the two groups; # P<0.05, significant difference between the baseline and days after operation."

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