北京大学学报(医学版) ›› 2014, Vol. 46 ›› Issue (3): 400-404.

• 论著 • 上一篇    下一篇

黑碳和臭氧处理的黑碳对小鼠遗传损伤的比较研究

郜鑫1,尚静2,杨敬林1,李前2,陈田1,庞元捷1,张文晓1,栾先国1,朱彤2,贾光1△   

  1. (1. 北京大学公共卫生学院劳动卫生与环境卫生学系, 北京100191; 2. 北京大学环境科学与工程学院, 北京100871)
  • 出版日期:2014-06-18 发布日期:2014-06-18

Comparison of genetic damage in mice exposed to black carbon and ozone-oxidized black carbon

GAO Xin1, SHANG Jin2, YANG Jing-in1, LI Qian2, CHEN Tian1, PANG Yuan-ie1, ZHANG Wen-iao1, LUAN Xian-uo1, ZHU Tong2, JIA Guang1△   

  1. (1. Department of Occupational and Environmental Health Sciences, Peking University School of Public Health, Beijing 100191, China; 2. College of Environmental Sciences and Engineering, Peking University, Beijing 100871, China)
  • Online:2014-06-18 Published:2014-06-18

摘要: 目的:评价大气黑碳(black carbon,BC)与臭氧处理黑碳(ozonized black carbon,O3- BC)对机体的遗传毒性,并从氧化应激的角度探讨其机制。方法:将74只体重为(28.0±1.5) g的雄性ICR小鼠随机分为7组,即磷酸盐缓冲液对照组和两种颗粒物的50 μg、100 μg、200 μg混悬液滴注组(每次每只动物分别滴注50、100、200 μg)。最高剂量组(200 μg组)为12只,其余组均为10只。染毒为每天气管滴注1次,连续滴注4天,末次染毒24 h后处死动物。测定血清过氧化氢酶(catalase,CAT)活性、肺匀浆丙二醛(malondialdehyde,MDA)含量、血清及随机尿8-羟基脱氧鸟苷(8-hydroxyguanosine,8-OHdG)水平,对骨髓涂片进行微核计数及肺组织病理分析。结果:小鼠一般情况良好,与对照组相比染毒组肝脏器系数降低(P<0.05);100 μg和200 μg两种颗粒物组血清CAT活性较对照组增加(P<0.05),微核率亦较对照组增加(P<0.05);各组血清及尿中8-OHdG和肺匀浆MDA差异无统计学意义;组织病理观察,暴露于颗粒物后肺组织出现炎症反应。结论:急性暴露于高剂量BC及O3-BC的小鼠体内氧化应激水平升高,并出现遗传损伤,但两种颗粒物毒性无明显差异,O3处理是否增加BC的遗传毒性还有待于进一步探讨。

关键词: 煤烟, 臭氧, 微核试验, 小鼠

Abstract: Objective:To make an assessment on the genotoxicity caused by black carbon (BC) and ozonized black carbon (O3-BC). Methods: In this study, 74 healthy male ICR mice [weighed (28±1.5) g] were randomly divided into 7 groups, including one phosphate buffer solution (PBS) control group and six particles exposed groups by intratracheal instillation with either BC or O3-BC at the doses of 50, 100, 200 μg/mouse, respectively. There were 12 mice in the groups of 200 μg/mouse and 10 mice in others. The mice were sacrificed 24 h after four intratrachealinstillations. The activities of catalase (CAT) in serum and the levels of malondialdehyde (MDA) in lung tissue homogenate were measured. As the DNA damage mark, 8-hydroxyguanosine (8-OHdG) in urine and serum were quantified with ELISA method. Micronucleus test was used for potential genotoxicity of BC and O3-BC. Hematoxylin and eosin staining was used to stain lung paraffin section. Results: The mice were in good condition during instillation, and the liver coefficient of the test groups was significantly lower than that of the control group (P<0.05). The activities of CAT in serum significantly increased in the 100 μg/mouse and 200 μg/mouse groups after being exposed to these two kinds of particles. The micronucleus rate in allthe BC and O3-BC exposed groups increased (P<0.05), but there was no statistically significant difference among the groups in the levels of 8-OHdG in serum and urine and MDA in lung tissue homogenate. Inflammatory response was found in the lung tissue under the microscope after exposure to BC and O3-BC. Conclusion: Intratracheal instillation of BC and O3-BC induced increasing of oxidative stress and genetic damage in mice. But there was no significant difference b

Key words: Soot, Ozone, Micronucleus tests, Mice

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