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北京大学学报（医学版） ›› 2020, Vol. 52 ›› Issue (1): 51-57. doi: 10.19723/j.issn.1671-167X.2020.01.008

• 论著 • 上一篇    下一篇

大鼠咬合干扰致口颌面痛敏的自我赏罚实验行为学特点

白珊珊1,2,莫思怡1,2,徐啸翔1,2,刘云1,2,谢秋菲1,2,△(),曹烨1,2,△()   

  1. 1. 北京大学口腔医学院·口腔医院, 修复科, 北京 100081
    2. 北京大学口腔医学院·口腔医院,口颌功能诊疗研究中心 国家口腔疾病临床医学研究中心 口腔数字化医疗技术和材料国家工程实验室 口腔数字医学北京市重点实验室, 北京 100081
  • 收稿日期:2019-10-10 出版日期:2020-02-18 发布日期:2020-02-20
  • 通讯作者: 谢秋菲,曹烨 E-mail:xieqiuf@163.com;ye.cao@bjmu.edu.cn
  • 基金资助:
    北京市自然科学基金(7192231)

Characteristics of orofacial operant test for orofacial pain sensitivity caused by occlusal interference in rats

Shan-shan BAI1,2,Si-yi MO1,2,Xiao-xiang XU1,2,Yun LIU1,2,Qiu-fei XIE1,2,△(),Ye CAO1,2,△()   

  1. 1. Center for Oral and Jaw Functional Diagnosis, Peking University School and Hospital of Stomatology & Department of Prosthodontics, Beijing 100081, China
    2. Center for Oral and Jaw Functional Diagnosis, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
  • Received:2019-10-10 Online:2020-02-18 Published:2020-02-20
  • Contact: Qiu-fei XIE,Ye CAO E-mail:xieqiuf@163.com;ye.cao@bjmu.edu.cn
  • Supported by:
    Supported by the Beijing Natural Science Foundation(7192231)

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摘要：

目的:测定咬合干扰模型(experimental occlusal interference, EOI)与部分眶下神经切断模型(partial infraorbital nerve transection,pIONX)两种口颌面疼痛模型大鼠的自我赏罚实验行为学表现及机械诱发反应痛敏,比较两种测痛方法所反映的不同疼痛模型的疼痛特点。方法:Sprague-Dawley大鼠随机分为8组,每组6只,分别为机械刺激诱发反应组(sham-EOI、EOI、sham-pIONX与pIONX组,sham为假手术组)与自我赏罚实验组(sham-EOI、EOI、sham-pIONX与pIONX组,sham为假手术组)。于建模前及建模后1、3、7、10、14、21 d测定各组大鼠机械刺激反应阈值与自我赏罚行为学表现。结果:机械刺激诱发反应组大鼠pIONX组von Frey纤维的机械刺激反应阈值于1~21 d出现显著下降(P<0.05),7~10 d达到最低;自我赏罚实验组大鼠pIONX组的总摄食时间于10~21 d出现显著下降(P<0.05),10~14 d达到最低。机械刺激诱发反应组大鼠EOI组von Frey纤维的机械刺激反应阈值于3~21 d出现显著下降(P<0.05),7 d达到最低;自我赏罚实验组大鼠EOI组的总摄食时间于1~21 d出现显著下降(P<0.05),7~10 d达到最低。结论:自我赏罚实验可以作为口颌面疼痛的行为学测定新方法,而且在神经病理性疼痛和咬合干扰所致口颌面疼痛的模型中均可稳定应用。两种模型中,自我赏罚实验与机械刺激诱发反应均表现出了不同的痛敏时程,两种方法互为补充可以更全面地揭示不同模型的疼痛行为学特点。

关键词: 动物行为, 面部疼痛, 痛觉过敏, 动物疾病模型, 大鼠

Abstract:

Objective: To compare the orofacial pain sensitivity with operant test and mechanical hyperalgesia with von Frey filaments of two orofacial pain models (EOI: experimental occlusal interference; pIONX: partial infraorbital nerve transection). To investigate the operant and evoked characteristics of EOI-rats. Methods: The orofacial operant behaviors were tested by Ugo Basile Orofacial Stimulation Test System. The mechanical thresholds of vibrissal pads were tested by von Frey filaments. Male Sprague-Dawley rats were randomly divided into eight groups: von Frey group: sham-EOI, EOI, sham-pIONX, pIONX (sham: sham-operated group); operant test group: sham-EOI, EOI, sham-pIONX, pIONX (sham: sham-operated group). The mechanical thresholds and orofacial operant behaviors were tested on pre-operation and post-operation days l, 3, 7, 10, 14 and 21. Results: In pIONX of von Frey group, the mechanical withdrawal threshold decreased from days 1 to 21 (P<0.05), peaking from days 7 to 10, and lasted until the end of the experiment. There was no significant difference between the bilateral sides. In pIONX of operant test group, the total contact time decreased from days 10 to 21 (P<0.05), peaking from days 10 to 14, and lasted until the end of the experiment. In EOI of von Frey group, the mechanical withdrawal threshold decreased from days 3 to 21 (P<0.05), peaking on day 7, and lasted until the end of the experiment. There was no significant difference between the bilateral sides. In EOI of operant test group, the total contact time decreased from days 1 to 21 (P<0.05), peaking from days 7 to 10, and lasting until the end of experiment. Conclusion: Orofacial operant test is a stable method to evaluate orofacial pain behaviors, which could discriminate the feature of neuropathic and EOI orofacial pain. In these two animal models, both of the operant behaviors and the mechanical hyperalgesia exhibited different time courses. Orofacial operant test provides a novel method for evaluating the orofacial pain sensitivity and studying the orofacial pain mechanism thoroughly.

Key words: Animal behavior, Facial pain, Hyperalgesia, Animal disease models, Rats

中图分类号: 

  • R782

图1

Ugo Basile面部疼痛测试仪"

图2

间断性禁食大鼠与自由饮食饮水大鼠的体质量变化(n=6)"

图3

sham-pIONX组与pIONX组大鼠双侧面部机械刺激反应阈值"

图4

sham-pIONX组与pIONX组大鼠自我赏罚行为学表现"

图5

sham-EOI组与EOI组大鼠双侧面部机械刺激反应阈值"

图6

sham-EOI组与EOI组大鼠自我赏罚行为学表现"

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ISSN 1671-167X
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