雌马酚对大肠癌细胞增殖的影响

doi:10.3969/j.issn.1671-167X.2017.03.003

北京大学学报(医学版) ›› 2017, Vol. 49 ›› Issue (3): 383-387. doi: 10.3969/j.issn.1671-167X.2017.03.003

雌马酚对大肠癌细胞增殖的影响

蔡源发，张华明，钮文异，邹永秋，马德福△

（北京大学公共卫生学院社会医学与健康教育系，北京100191）

出版日期:2017-06-18 发布日期:2017-06-18
通讯作者: 马德福 E-mail: madefu@bjmu.edu.cn
基金资助:
国家自然科学基金(81202193，81573130)、北京市自然科学基金(7122103，7172117)资助

Effects of equol on colon cancer cell proliferation

CAI Yuan-fa, ZHANG Hua-ming, NIU Wen-yi, ZOU Yong-qiu, MA De-fu△

(Department of Social Medicine and Health Education, Peking University School of Public Health, Beijing 100191, China)

Online:2017-06-18 Published:2017-06-18
Contact: MA De-fu E-mail: madefu@bjmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China (81202193，81573130) and the Beijing Natural Science Foundation(7122103，7172117)

摘要/Abstract

摘要： 目的：检测大豆异黄酮代谢产物雌马酚对大肠癌细胞增殖的影响，并探讨其分子作用机制。方法：培养大肠癌细胞 DLD1、HCT15、COLO205、LOVO和SW480，用3-(4，5-二甲基噻唑-2)-2，5-二苯基四氮唑溴盐［3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide，MTT］法检测雌马酚对大肠癌细胞增殖的影响，用逆转录PCR和蛋白免疫印迹法分别检测雌激素受体和核因子红系2相关因子2［nuclear factor (erythroidderived 2)-like 2，Nrf2］表达状况。用MTT法检测雌激素受体抑制剂和激动剂对大肠癌细胞增殖的影响。结果：DLD1、HCT15、COLO205、LOVO和SW480均表达雌激素受体（estrogen receptor, ER）β，但只有DLD1和HCT15有ERα弱表达。MTT试验显示不同剂量雌马酚（0、0.5、1、5、10 μmol/L）不仅能够显著抑制ERα和ERβ均表达的大肠癌细胞HCT-15的增生，且呈现明显的剂量效应关系，而且能够抑制只有ERβ表达的大肠癌细胞LOVO和SW480的增生，且呈现明显的剂量效应关系。此外，逆转录PCR发现不同剂量雌马酚刺激后，HCT-15的雌激素受体ERα和ERβ表达明显增加，呈剂量效应关系。蛋白免疫印迹法进一步证实雌激素受体ERα和ERβ蛋白表达随雌马酚剂量的增加而增加，蛋白免疫印迹法发现Nrf2随干预剂量的增加而表达明显增加。采用ERβ激动剂、雌激素受体抑制剂和特异性雌激素受体ERα激动剂对SW480、LOVO、HCT-15细胞刺激后，只发现不同剂量ERβ激动剂能够显著抑制大肠癌细胞SW480、LOVO、HCT-15的增生，且呈明显的剂量效应关系，但是，雌激素受体抑制剂和特异性雌激素受体ERα激动剂对SW480、LOVO和HCT-15细胞没有呈现显著的影响。结论：雌马酚能够通过雌激素样作用和抗氧化活性抑制大肠癌细胞的增殖。

关键词: 雌马酚, 大肠癌, 雌激素受体, 增殖

Abstract: Objective：To investigate the effect of equol on the proliferation of colom cancer cells and to explore the mechanisms. Methods: Colon cancer cells (DLD1,HCT15,COLO205,LOVO,SW480) were incubated, the cell proliferation was identified by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay. Reverse transcription PCR and Western blot were used to measure the mRNA and the protein expression of estrogen receptor and nuclear factor (erythroid-derived 2)-like 2 (Nrf2)in the colon cancer cells, respectively. Moreover, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay was used to investigate the effect of estrogen receptor(ER) inhibitor,ERα agonist, and estrogen receptor ERβagonist on the cell proliferation. Results: ERα was faintly expressed in the DLD-1 and HCT-15 cells. However, ERβ expression in DLD1, HCT15, COLO205, LOVO, and SW480 colon cancer cells. Different concentrations of equol (0, 0.5, 1, 5, 10 μmol/L) significantly inhibited the growth of HCT-15 cell with the expression of ERα and ERβ.More-over, different concentrations of equol (0, 0.5, 1, 5, 10 μmol/L) significantly inhibited the growth of LOVO, and SW480 cells with the ERβ expression in a dosedependent manner as demonstrated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay. mRNA expressions of ERα and ERβ in HCT-15 were stimulated significantly. Western blotting proved that the protein expressions of ERα and ERβ increased with the increasing of equol dose. Moreover we found significant difference of Nrf2 protein expression in HCT-15 cell stimulated by different concentrationss of equol. After the similation of estrogen receptor inhibitor, ERα agonist, or ERβ agonist, we found that only dif-ferent concentrations of ERβ agonist(0, 1, 10, 100, 1 000, 10 000 nmol/L) significantly inhibited the growth of HCT-15, LOVO, and SW480 in adose-dependent manner. Estrogen receptor inhibitor and ERα agonistdid not present significant effect on the cell proliferation of HCT-15, LOVO, and SW480. Conclusion: Equol inhibited the colon cancer cell proliferation by its estrogenic activities and antioxidant activities.

Key words: Equol, Colon cancer, Estrogen receptor, Proliferation

中图分类号:

蔡源发，张华明，钮文异，邹永秋，马德福. 雌马酚对大肠癌细胞增殖的影响[J]. 北京大学学报(医学版), 2017, 49(3): 383-387.

CAI Yuan-fa, ZHANG Hua-ming, NIU Wen-yi, ZOU Yong-qiu, MA De-fu. Effects of equol on colon cancer cell proliferation[J]. Journal of Peking University(Health Sciences), 2017, 49(3): 383-387.

参考文献

相关文章 15

[1]	杨朵,周心娜,王硕,王小利,袁艳华,杨化兵,耿会珍,彭兵,李子博,李彬,任军. 树突状细胞疫苗特异肿瘤多肽联合树突状细胞体外刺激淋巴细胞功能评估[J]. 北京大学学报（医学版）, 2021, 53(6): 1094-1098.
[2]	王子乔,刘燕鹰,张霞,刘田,任立敏,沈丹华,王屹,栗占国. 17例误诊为IgG4相关疾病患者的临床特点及误诊原因分析[J]. 北京大学学报（医学版）, 2019, 51(6): 1025-1031.
[3]	谢静,赵玉鸣,饶南荃,汪晓彤,方滕姣子,李晓霞,翟越,李静芝,葛立宏,王媛媛. 3种口腔颌面部来源的间充质干细胞成血管内皮分化潜能的比较研究[J]. 北京大学学报（医学版）, 2019, 51(5): 900-906.
[4]	张帆,燕太强,郭卫. Rasfonin抑制骨肉瘤细胞143B的增殖和迁移[J]. 北京大学学报（医学版）, 2019, 51(2): 234-238.
[5]	季兰岚,郝燕捷,张卓莉. 原发性骨髓纤维化引起的继发性痛风1例[J]. 北京大学学报（医学版）, 2018, 50(6): 1117-1119.
[6]	王子成，程立，吕同德，苏黎，林健，周利群. 炎症因子预处理的脂肪干细胞可明显抑制外周血单个核细胞增殖[J]. 北京大学学报(医学版), 2018, 50(4): 590-594.
[7]	唐旭，赵卫红，宋琴琴，殷华奇，杜依青，盛正祚，王强，张晓威，李清，刘士军，徐涛. SOX10对前列腺癌细胞增殖及侵袭的影响[J]. 北京大学学报(医学版), 2018, 50(4): 602-606.
[8]	汪晓彤，饶南荃，方腾姣子，赵玉鸣，葛立宏. 乳牙牙髓干细胞CD146阳性/阴性细胞亚群生物学特性的比较[J]. 北京大学学报(医学版), 2018, 50(2): 284-292.
[9]	陈玮, 胡凡磊, 刘洪江, 徐丽玲, 李英妮, 栗占国. 类风湿关节炎患者髓系来源的抑制细胞促进自身B细胞增殖[J]. 北京大学学报(医学版), 2017, 49(5): 819-823.
[10]	蔡燚，郭浩，李汉忠，王文达，张玉石. 结节性硬化症细胞株TSC2-/- MEFs和正常细胞株TSC2+/+ MEFs微小RNA表达谱的差异分析[J]. 北京大学学报(医学版), 2017, 49(4): 580-584.
[11]	杨迪，许珺辉，邓芙蓉，郭新彪. 纳米银对皮肤细胞半通道活性的影响及其在细胞增殖抑制中的作用[J]. 北京大学学报(医学版), 2017, 49(3): 371-375.
[12]	高翔，陈香梅，张婷，张静，陈茉，郭正阳，石岩岩，鲁凤民，丁士刚. 巨噬细胞加帽蛋白与胃癌细胞增殖及迁移能力的关系[J]. 北京大学学报(医学版), 2017, 49(3): 489-494.
[13]	司马梓涵，洪瑛瑛，李铁军. PTCH1基因突变对牙源性角化囊性瘤上皮细胞增殖的影响[J]. 北京大学学报(医学版), 2017, 49(3): 522-526.
[14]	隋华欣，吕培军，王宇光，王勇，孙玉春. 低能量激光照射对人脂肪基质细胞增殖分化的影响[J]. 北京大学学报(医学版), 2017, 49(2): 337-343.
[15]	李静文，殷晓晖，栾庆先. 不同代次诱导多能干细胞增殖及牙周定向分化的能力[J]. 北京大学学报(医学版), 2017, 49(1): 16-024.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

雌马酚对大肠癌细胞增殖的影响

Effects of equol on colon cancer cell proliferation

RICH HTML

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10