北京大学学报(医学版) ›› 2014, Vol. 46 ›› Issue (3): 347-354.

• 论著 • 上一篇    下一篇

孕期和哺乳期槲皮素干预对肥胖母鼠后代的体重及胰岛素样生长因子-1 mRNA表达的影响

徐昊1,吴峥昊1,许雅君1, 2△   

  1. (1. 北京大学公共卫生学院营养与食品卫生学系,北京100191; 2. 食品安全毒理学研究与评价北京市重点实验室,北京100191)
  • 出版日期:2014-06-18 发布日期:2014-06-18

Effects of quercetin in pregnant and lactation period on weight and expression of insulin-like growth factors-1 mRNA of obese female rats offspring

XU Hao1, WU Zheng-hao1, XU Ya-jun1, 2△   

  1. (1.Department of Nutrition and Food Hygiene, Peking University School of Public Health, Beijing 100191, China; 2.Beijing Key Laboratory of Toxicological Research and Risk Assessment for Food Safety, Beijing 100191, China)
  • Online:2014-06-18 Published:2014-06-18

摘要: 目的:研究在孕期及哺乳期肥胖母鼠进行槲皮素干预后, 对其F1代仔鼠体重和胰岛素样生长因子-1(insulin-like growth factors-1, IGF-1)mRNA表达的影响。方法:健康雌性SD大鼠断乳后,随机抽取8只喂养基础饲料作为空白对照组,其余喂养高脂饲料。当高脂饲料组大鼠平均体重超过空白对照组大鼠平均体重20%时,认为造模成功。将造模成功后的母鼠与健康雄鼠交配受孕,肥胖孕鼠随机分成4组,每组8只,整个孕期和哺乳期喂食高脂饲料,并分别以0 mg/kg体重(高脂对照组)、50 mg/kg体重、100 mg/kg体重、200 mg/kg体重剂量的槲皮素灌胃;空白对照组整个实验期喂食基础饲料并以溶剂灌胃。各组孕鼠自然分娩,记录F1代仔鼠出生体重及生后d7,d21,d56和d98体重,并检测F1代仔鼠肝组织IGF-1 mRNA的表达水平。结果:高脂对照组F1代仔鼠出生体重显著高于空白对照组。随槲皮素干预剂量增大,高脂对照组F1代仔鼠出生体重逐渐降低,接近空白对照组水平。槲皮素在一定程度上抑制F1代仔鼠肝组织IGF-1 mRNA的表达,持续到成年期,200 mg/kg体重效果最显著。结论:肥胖孕鼠F1代仔鼠出生体重显著增加,而孕期槲皮素干预可有效逆转F1代仔鼠体重,并控制F1代仔鼠断乳前体重过快增长。200 mg/kg体重的槲皮素能够有效抑制F1代仔鼠IGF-1 mRNA的表达,从而抑制细胞过度增殖和分化。

关键词: 槲皮素, 体重, 胰岛素样生长因子-1, 肥胖症, 大鼠

Abstract: Objective: To study the effect of quercetin given during pregnancy and lactation period of obese rats on weight and expression of insulin-like growth factors-1 (IGF-1) mRNA of the offspring.Methods: In the study, 8 healthy weaning female SD rats were randomly selected to feed the basal diet and as blank control group, and the others were fed with high-fat diet. When the average weight of the high-fat diet rats was 20% as many as the average weight of the control group, we believed the model succeeded. Then the female SD rats were mated and the pregnant rats were randomly divided into 5 groups, 8 rats in each. They were fed with high fat diet during pregnancy and lactation, and the obese pregnant rats were respectively irrigated with 0 mg/kg body weight (high-fat control), 50 mg/kg body weight,100 mg/kg body weight, and 200 mg/kg body weight quercetin. The blank control group was fed with basic diet throughout the experimental period. The birth weight, the weights during the development of d7, d21, d56, and d98, and the expressions of IGF-1 mRNA in livers tissue of the F1 generation were measured.Results: The average birth weight of the F1 generation of high fat diet (HFD) group was significantly higher than that of the blank control group. Decreased offspring birth weight was observed with the intervention of quercetin, which effect remained to the delectation. The quercetin also reduced the expression of IGF-1 mRNA in livers of the F1 generation, and this effect remained to the adulthood. The 200 mg/kg body weight quercetin was most significantly effective.Conclusion: The averaged birth weight of the F1 generation of obese pregnant rats was significantly increased, and quercetin could effectively inhibit the expression of IGF-1 mRNA in livers of F1 generation to decrease cell proliferation and cell differentiation.

Key words: Quercetin, Body weight, Insulin-like growth factor-1, Obesity, Rats

[1] 李卫民,祖菲娅·吐尔地. 维吾尔族、哈萨克族和汉族大学生体重指数与体能指数的关系[J]. 北京大学学报(医学版), 2024, 56(3): 411-417.
[2] 张警丰,金银姬,魏慧,姚中强,赵金霞. 体重指数与类风湿关节炎临床特征的相关性分析[J]. 北京大学学报(医学版), 2023, 55(6): 993-999.
[3] 张展奕,张帆,颜野,曹财广,李长剑,邓绍晖,孙悦皓,黄天亮,管允鹤,李楠,陆敏,胡振华,张树栋. 近红外荧光靶向探针用于前列腺神经血管束术中成像[J]. 北京大学学报(医学版), 2023, 55(5): 843-850.
[4] 孟令玮,李雪,高胜寒,李悦,曹瑞涛,张毅,潘韶霞. 三种方法建立大鼠种植体周炎模型的比较[J]. 北京大学学报(医学版), 2023, 55(1): 22-29.
[5] 何伟,杨思雯,陈娟,朱晓俊,陈志忠,马文军. 275 nm和310 nm紫外线对去卵巢骨质疏松大鼠骨代谢的影响[J]. 北京大学学报(医学版), 2022, 54(2): 236-243.
[6] 陈章健,韩硕,郑湃,贾光. 锐钛矿型纳米二氧化钛经口暴露90天对Sprague-Dawley大鼠血常规指标的影响[J]. 北京大学学报(医学版), 2021, 53(6): 1205-1208.
[7] 王贵红,左婷,李然,左正才. 瑞巴派特在大鼠痛风性关节炎急性发作中的作用[J]. 北京大学学报(医学版), 2021, 53(4): 716-720.
[8] 尹雪倩, 张晓玄, 文婧, 刘思奇, 刘欣然, 周若宇, 王军波. 荞麦、燕麦、豌豆复配对糖尿病大鼠血糖的影响[J]. 北京大学学报(医学版), 2021, 53(3): 447-452.
[9] 白枫,何倚帆,牛亚楠,杨若娟,曹静. 超细颗粒物对大鼠离体灌注心脏功能的影响[J]. 北京大学学报(医学版), 2021, 53(2): 240-245.
[10] 史欣然,安美静,陈天娇,马军. 饮奶行为在家庭社会经济状况与儿童青少年体重指数间的中介作用[J]. 北京大学学报(医学版), 2021, 53(2): 308-313.
[11] 周迪,陈章健,胡贵平,阎腾龙,龙昌茂,冯慧敏,贾光. 纳米二氧化钛亚急性经口暴露对大鼠氧化/抗氧化生物标志和炎性因子的影响[J]. 北京大学学报(医学版), 2020, 52(5): 821-827.
[12] 候越,赵旭彤,谢志颖,袁云,王朝霞. 线粒体DNA 8344 A>G突变导致的MELAS/MERRF/Leigh重叠综合征[J]. 北京大学学报(医学版), 2020, 52(5): 851-855.
[13] 陈章健,韩硕,郑湃,周淑佩,贾光. 纳米二氧化钛与葡萄糖亚慢性联合经口暴露对幼年大鼠血清叶酸和维生素B12水平的影响[J]. 北京大学学报(医学版), 2020, 52(3): 451-456.
[14] 韩硕,陈章健,周迪,郑湃,张家赫,贾光. 纳米二氧化钛经口暴露90天对大鼠粪便代谢组的影响[J]. 北京大学学报(医学版), 2020, 52(3): 457-463.
[15] 张晓圆,郭成成,玉应香,谢岚,常翠青. 高脂饲料诱导肥胖胰岛素抵抗大鼠模型的建立[J]. 北京大学学报(医学版), 2020, 52(3): 557-563.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!