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Journal of Peking University(Health Sciences) ›› 2018, Vol. 50 ›› Issue (6): 1083-1087. doi: 10.19723/j.issn.1671-167X.2018.06.025

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Staining effect of bovine incisors in vitro by tea solutions

Meng-meng ZHU1,Ying-long LI2,Jie PAN1,△()   

  1. 1. Department of General, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
    2. Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
  • Received:2017-10-09 Online:2018-12-18 Published:2018-12-18
  • Contact: Jie PAN E-mail:panjie72@sina.com
  • Supported by:
    Supported by the National Natural Science Foundation of China(81200821)

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Abstract:

Objective: To determine the optimum staining condition of tea solutions on bovine incisors in vitro, by comparing the color stability of tooth surface of different concentrations of tea solutions and methods on bovine incisors in vitro.Methods:Twenty bovine incisors with color surface A1 were chosen, then randomly divided into 4 groups (n=10). Group 1: soaked with 2% tea solution continuously for 6 days; group 2: soaked with 2% tea solution for 6 days, but changed fresh tea solution everyday; group 3: soaked with 1% tea solution continuously for 6 days; group 4: soaked with 1% tea solution for 6 days but fresh tea solution changed every day. After 6 days of staining, the surface color (ΔE value)of all the samples were measured with crystal eye. After brushing 30 times with toothbrushes, the color of bovine incisors were measured again. Then the samples were soaked in artificial saliva at 37 ℃, and ΔE value was measured for 14 days.Results:After staining for 6 days, the ΔE values of the 2% tea solution groups were better than those of the 1% groups (20.21 vs. 16.44, 24.09 vs. 19.22, P<0.05);the groups with the same tea solution concentration, a better result was observed for the group soaked with daily fresh tea solution than for the group that experienced continuous staining (24.09 vs. 20.21, 19.22 vs. 16.44, P<0.05).Groups 1 and 2 were selected for subsequent brushing experiments. The color of both groups became lighter after brushing, and a better result was observed for the continuous staining group than for the group stained in daily fresh solution (3.06 vs. 9.51, P<0.05). The samples with better coloring effect soaked with 2% tea solution continuously for 6 days were put into artificial saliva for 14 days. There was not any significant change in coloring at theend of the first two days (1.51 vs. 1.51, P>0.05), and the color was visibly lighter after the third day (1.51 vs. 5.89, P<0.05), and no further significant change was observed until the 14th day (5.81 vs.5.89, P>0.05), which was darker coloring than that of the pre-staining group.Conclusion:Continuous staining on bovine incisors with 2% tea solution with subsequent soaking in artificial saliva resulted in consistent coloring from day 3 to day 14, and this method could be used as an ideal model for teeth staining in vitro.

Key words: Tea solution, Staining effect, Bovine incisor

CLC Number: 

  • R781

Figure 1

Staining effect of different concentrations of tea solutions and different methodsThe ΔE value of four groups before and after staining are: 20.21, 24.09, 16.44, 19.22. Group 1, 3 (P=0.01), group 2, 4 (P=0.00), group 1, 2 (P=0.01) and group 3, 4 (P=0.04) all have statistical significance (P< 0.05, F=11.41). With the same staining method, 2% group was better than 1% group. Besides, a better result was observed for the group with fresh tea solution every day than the group with continuous staining.*P<0.05."

Figure 2

Staining effects after brushing with different solutionsThe initial value and ΔE value for group 1 with 2% continuously staining before and after brushing are 20.21, 17.15, the P value is 0.04, the difference was statistically significance (P<0.05, F=5.09). The ΔE value for group 2 with 2% daily fresh solution groupis 24.09, 14.58, which had a statistically significance (P<0.05, F=38.09). The P value for the comparison of the ΔE values after brushing is 0.04, which had a statistically significance (P<0.05, F=4.50).After brushing, a better result was observed for group 1 with continuous staining group.*P<0.05, statistically significant."

Figure 3

Coloring difference after brushing for samples soaked in artificial saliva for 14 daysThe samples soaked in artificial saliva for day 1 and day 2 didn’t have significant decrease after brushing (ΔE<2.75,P>0.05), and the color became significantly lighter atday 3 (ΔE>2.75,P<0.05). No significant color change was observed from day 3 to day 14 (P>0.05). This indicates that the stable coloring effect was acquired from day 3 to day 14 soaked in artificial saliva."

[1] Rajesh AG, Ranganath LM, Kumar KS , et al. Surface morpho-logical changes in human enamel following bleaching: an in vitro scanning electron microscopic study[J]. J Contemp Dent Pract, 2012,13(3):405-415.
doi: 10.5005/jp-journals-10024-1159 pmid: 22918018
[2] Xing W, Jiang T, Liang S , et al. Effect of in-office bleaching agents on the color changes of stained ceromers and direct com-posite resins[J]. Acta Odontol Scand, 2014,72(8):1032-1038.
doi: 10.3109/00016357.2014.946962 pmid: 25196263
[3] Lee BS, Huang SH, Chiang YC , et al. Development of in vitro tooth staining model and usage of catalysts to elevate the effectiveness of tooth bleaching[J]. Dent Mater, 2008,24(1):57-66.
doi: 10.1016/j.dental.2007.01.012 pmid: 17442388
[4] Lee RJ, Bayne A, Tiangco M , et al. Prevention of tea-induced extrinsic tooth stain[J]. Int J Dent Hyg, 2014,12(4):267-272.
doi: 10.1111/idh.12096 pmid: 25040739
[5] Agnihotry A, Gill KS, Singhal D , et al. A Comparison of the bleaching effectiveness of chlorine dioxide and hydrogen peroxide on dental composite[J]. Braz Dent J, 2014,25(6):524-527.
doi: 10.1590/0103-6440201300098 pmid: 25590199
[6] Homayoom A, Sadat HKS, Nafiseh Z , et al. In vitro effect of casein phosphopeptide amorphous calcium phosphate on enamel susceptibility to staining by tea during bleaching treatment[J]. J Dent, 2015,12(8):607-613.
pmid: 27123021
[7] Çelik C, Yüzügüllü B, Erkut S , et al. Effect of bleaching on staining susceptibility of resin composite restorative materials[J]. J Esthet Restor Dent, 2010,21(6):407-414.
doi: 10.1111/j.1708-8240.2009.00299.x pmid: 20002929
[8] Suliemana M, Addya M, Macdonalda E , et al. The bleaching depth of a 35% hydrogen peroxide based in-office product: a study in vitro[J]. J Dent, 2005,33(1):33-40.
doi: 10.1016/j.jdent.2004.07.008
[9] Sulieman M, Addy M, Rees JS . Development and evaluation of a method in vitro to study the effectiveness of tooth bleaching[J]. J Dent, 2003,31(6):415-422.
doi: 10.1016/S0300-5712(03)00069-1 pmid: 12878024
[10] 路丽 . 着色牙外漂白后再矿化的效果及对细菌粘附影响的体外实验研究[D]. 山东: 山东大学, 2005.
[11] 李艳 . 三种着色牙模型漂白前后颜色的比较研究[D]. 湖南: 中南大学, 2012.
[12] Odaira C, Itoh S, Ishibashi K . Clinical evaluation of a dental color analysis system: the crystaleyespectrophotometer[J]. J Prosthodont Res, 2011,55(4):199-205.
doi: 10.1016/j.jpor.2010.12.005 pmid: 21296639
[13] Wang C, Li Y, Wang X , et al. The enamel microstructures of bovine Omandibular incisors[J]. Anat Rec, 2012,295(10):1698-1706.
doi: 10.1002/ar.22543 pmid: 22837182
[14] Addy M, Moran J . Mechanism of stain formation on teeth, in particular associated with metal ions and antiseptics[J]. Adv Dent Res, 1995,9(4):450-456.
doi: 10.1177/08959374950090041601
[15] Carpenter GH, Pramanik R, Proctor GB . An in vitro model of chlorhexidine-induced tooth staining[J]. J Periodental Res, 2005,40(3):225-230.
doi: 10.1111/j.1600-0765.2005.00791.x pmid: 15853968
[16] Berger SB, Coelho AS, Oliveira VA , et al. Enamel susceptibility to red wine staining after 35% hydrogen peroxide bleaching[J]. J Appl Oral Sci, 2008,16(3):201-204.
doi: 10.1590/S1678-77572008000300007
[17] Phan X, Akyalcin S, Wiltshire WA , et al. Effect of tooth bleaching on shear bond strength of a fluoride- releasing sealant[J]. Angle Orthod, 2012,82(3):546-551.
doi: 10.2319/052711-353.1 pmid: 21854189
[18] Joiner A, Muller D, Elofsson UM , et al. Ellipsometry analysis of the in vitro adsorption of tea polyphenols onto salivary pellicles[J]. Eur J Oral Sci, 2004,112(6):510-515.
doi: 10.1111/j.1600-0722.2004.00166.x pmid: 15560834
[19] Pontefract H, Courtney M, Smith S , et al. Development of methods to enhance extrinsic tooth discoloration for comparison of toothpastes I studies in vitro[J]. J Clin Periodontol, 2004,31(1):1-6.
doi: 10.1111/cpe.2004.31.issue-1
[20] Li Q, Xu BT, Li R , et al. Quantitative evaluation of colour regression and mineral content change of bleached teeth[J]. J Dent, 2010,38(3):253-260.
doi: 10.1016/j.jdent.2009.11.005 pmid: 19945498
[21] 李青, 王贻宁, 徐东选 . 四种常见离体牙保存液对牙齿颜色的影响. 口腔医学研究, 2005,21(2):197-199.
doi: 10.3969/j.issn.1671-7651.2005.02.027
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