关键词: 光谱法, 质量, 基质辅助激光解吸电离, 链球菌, 变异, 龋齿, 唾液

Abstract: Objective：To identify Streptococcus mutans (S. mutans) in carious patients’ saliva using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), and to establish a faster and more accurate method to identify S. mutans. Methods:In this study, a total of 90 carious patients from Department of Endodontics of Peking University School of Stomatology were recruited. All these patients’ saliva was collected. After extracting the protein of the samples, they were identified by MALDI-TOF MS. The composite profile was analyzed using BioExplorer 1.0 software. The scores≥25 were considered as S. mutans, whereas the scores <25 were as considered as non S. mutans. Finally, these results were compared with 16S rDNA sequencing to figure out the sensitivity and concordance rate, respectively. Results:The sensitivity of MALDI-TOF MS was 96.0%, and the concordance rate compared with 16S rDNA sequencing was as high as 98.7%. Conclusion:MALDI-TOF MS is high throughput, rapid and easy to perform in comparison to other conventional methods. It has a high sensivity and concordance rate. Thus, MALDI-TOF MS can serve as an effective tool for identification of S. mutans.

Key words: Spectrometry, mass, matrix-assisted laser desorption-ionization, Streptococcus mutans, Dental caries, Saliva