北京大学学报(医学版) ›› 2017, Vol. 49 ›› Issue (3): 394-402. doi: 10.3969/j.issn.1671-167X.2016.03.005

• 论著 • 上一篇    下一篇

大气颗粒物及多环芳烃暴露与慢性阻塞性肺疾病患者全身性氧化应激水平

张怡1,宋晓明1,赵茜1,王童1,李丽娟2,陈婕1,徐洪兵1,刘贝贝3,孙晓燕3,贺蓓3,#br# 黄薇1△   

  1. (1. 北京大学公共卫生学院劳动卫生与环境卫生学系, 北京100191; 2. 中国科学院地球环境研究所, 西安 710061; 3. 北京大学第三医院呼吸科, 北京100191)
  • 出版日期:2017-06-18 发布日期:2017-06-18
  • 通讯作者: 黄薇 E-mail:whuang@bjmu.edu.cn
  • 基金资助:
    国家自然科学基金(21190051)资助

Effects of exposure to ambient particulate matter and polycyclic aromatic hydrocarbons on oxidative stress biomarkers in the patients with chronic obstructive pulmonary disease

ZHANG Yi1, SONG Xiao-ming1, ZHAO Qian1, WANG Tong1, Li Li-juan2, CHEN Jie1, XU Hong-bing1, LIU Bei-bei3, SUN Xiao-yan3, HE Bei3, HUANG Wei1△   

  1. (1. Department of Occupational and Environmental Health, Peking University School of Public Health, Beijing 100191, China; 2. Institute of Earth Environment, Chinese Academy of Sciences, Xi’an 710061, China; 3. Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100191, China)
  • Online:2017-06-18 Published:2017-06-18
  • Contact: HUANG Wei E-mail:whuang@bjmu.edu.cn
  • Supported by:
    Supported by National Natural Science Foundation of China (21190051)

摘要:  目的:研究环境大气颗粒物及多环芳烃(polycyclic aromatic hydrocarbons, PAHs)暴露对慢性阻塞性肺疾病(chronic obstructive pulmonary disease, COPD)患者全身性氧化应激水平的影响。方法:招募45名居住在北京大学医学部半径5 km范围内的COPD患者作为研究对象,采用定组研究方法,于2014年11月至2015年5月对研究对象进行两次临床随访。通过肺功能检查测定第1秒用力呼气容积占预计值百分比(即FEV1%预计值),用于评估COPD患者病情的严重程度。收集患者尿样,分别采用高效液相色谱(high performance liquid chromatography, HPLC)法和酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)法测定尿样中的全身性氧化应激指标丙二醛(malondialdehyde, MDA)和8-羟基脱氧鸟苷(8-hydroxyl-2′-deoxyguanosine, 8-OHdG)水平,同时,通过本课题组于校园内自行建立的空气污染监测站点连续收集研究期间该区域的环境大气污染物浓度以获得人群污染物暴露水平。利用线性混合效应模型,分别通过单污染物模型、双污染物模型和分层分析来研究大气污染物对COPD患者尿样中MDA和8-OHdG水平的影响。结果:滞后2 d(lag2)的超细颗粒物(ultrafine particles, UFP)和PAHs浓度与尿样中MDA水平呈显著正相关(P<0.05),UFP和PAHs每升高四分位间距(interquartile range, IQR)浓度,MDA浓度分别升高28%(95%CI: 4%~57%)和36%(95%CI: 4%~77%), 控制黑碳(black carbon, BC)的影响后,UFP和PAHs与MDA的关联强度略有升高。以COPD的严重程度进行分层分析后发现,多数污染物在疾病程度较轻的COPD患者中具有更强的氧化应激效应。在FEV1%预计值≥50%的COPD患者中发现,UFP每升高IQR浓度,尿样中MDA浓度升高98%(95%CI: 38%~186%),BC、UFP和PAHs每升高IQR浓度,尿样中8-OHdG浓度分别升高87%(95%CI: 32%~166%)、69%(95%CI: 24%~130%)和156%(95%CI: 66%~294%)。本研究未观察到细颗粒物(fine particulate matter, PM2.5)与尿样中氧化应激指标的显著关联。结论:大气污染物暴露可加重COPD患者全身性氧化应激水平,其中UFP和PAHs的效应更为显著,并且污染物对于疾病程度较轻的COPD患者氧化应激效应更强。

关键词: 空气污染, 颗粒物, 多环芳烃, 氧化应激, 肺疾病, 慢性阻塞性

Abstract: Objective:To investigate the effects of exposure to ambient particulate matter (PM) and polycyclic aromatic hydrocarbons (PAHs) on systemic oxidative stress biomarkers in chronic obstructive pulmonary disease (COPD) patients. Methods: A panel of forty-five diagnosed and stable COPD patients, whose residences were within 5 kilometers from Peking University Health Science Center (PKUHSC), were recruited and followed up twice between November 2014 and May 2015. The lung function index percentage of forced expiratory volume in 1 second (FEV1) to predicted value (FEV1%pred), was measured to reflect the severity of COPD patients. The systemic oxidative stress biomarkers malondialdehyde (MDA) and 8-hydroxyl-2′-deoxyguanosine (8-OHdG) in their urine samples were measured using high performance liquid chromatography (HPLC) and enzyme-linked immunosorbent assay (ELISA), respectively. Concentrations of ambient air pollutants and levels of meteorological factors were measured continuously through the air pollution-monitoring station located in PKUHSC. PM2.5 samples, which were used for measuring the concentrations of PAHs, were collected by PM2.5 high volume air sampler. We constructed linear mixed-effects models, including single-pollutant model, 2-pollutant models and stratification analysis, to estimate the effects of air pollutants on urinary MDA and 8-OHdG after adjusting for confounding factors. Results: In our COPD-patient panel, the associations between ultrafine particles (UFP) and PAHs and urinary MDA were statistically significant at lag2 (P<0.05). For an interquartile range (IQR) increase in UFP and PAHs, respective increases of 28% (95%CI: 4%-57%) and 36% (95%CI: 4%-77%) in urinary MDA were observed, and the effects became stronger after adjusting for the concentration of black carbon (BC). The COPD patients were divided into 2 groups stratified by FEV1%pred. Most air pollutants had stronger effects of systemic oxidative stress in the COPD patients of FEV1%pred≥50%. In this group, we observed that an IQR increase in UFP was associated with a 98% (95%CI: 38%-186%) increase in urinary MDA, and an IQR increase in BC, UFP and PAHs were associated with respective increases of 87% (95%CI: 32%-166%), 69% (95%CI: 24%-130%) and 156% (95%CI: 66%-294%) in urinary 8-OHdG. We didn’t find significant associations between fine particulate matter (PM2.5) and urinary oxidative stress biomarkers. Conclusion: Our results suggested that exposure to air pollutants, especially UFP and PAHs, was responsible for exacerbation of systemic oxidative stress in COPD patients. Most air pollutants had stronger effects of systemic oxidative stress in mild to moderate COPD patients.

Key words: Air pollution, Particulate matter, Polycyclic aromatic hydrocarbons, Oxidative stress, Pulmonary disease, chronic obstructive

中图分类号: 

  • R122
[1] 阎腾龙,胥嘉钰,陈田,杨鑫,王伟伟,周淑佩,牛丕业,贾光,夏交. 亚慢性PM2.5和O3共同暴露对大鼠鼻黏膜ATP总量及ATP酶活性的影响[J]. 北京大学学报(医学版), 2024, 56(4): 687-692.
[2] 周庆欣,杨晴晴,石舒原,李沛,孙凤. 健康体检人群血尿酸与气流阻塞的相关性[J]. 北京大学学报(医学版), 2024, 56(4): 693-699.
[3] 庞博,郭桐君,陈曦,郭华棋,石嘉章,陈娟,王欣梅,李耀妍,单安琪,余恒意,黄婧,汤乃军,王艳,郭新彪,李国星,吴少伟. 天津与上海35岁以上人群氮氧化物个体暴露水平及其影响因素[J]. 北京大学学报(医学版), 2024, 56(4): 700-707.
[4] 郭煌达,彭和香,王斯悦,侯天姣,李奕昕,章涵宇,王梦莹,武轶群,秦雪英,唐迅,李劲,陈大方,胡永华,吴涛. 短期大气颗粒物暴露和MTNR1B基因多态性对甘油三酯-葡萄糖指数影响的家系研究[J]. 北京大学学报(医学版), 2024, 56(3): 375-383.
[5] 李文根,古晓东,翁锐强,刘苏东,陈超. 血浆外泌体miR-34-5p和miR-142-3p在系统性硬化症中的表达及临床意义[J]. 北京大学学报(医学版), 2023, 55(6): 1022-1027.
[6] 王子恺,莫佳丽,张蒙,廖纪萍. 2013—2020年北京市慢性阻塞性肺疾病急性加重女性住院患者的流行病学和住院费用分析[J]. 北京大学学报(医学版), 2023, 55(6): 1074-1081.
[7] 刘志伟,刘鹏,孟凡星,李天水,王颖,高嘉琪,周佐邑,王聪,赵斌. 内源性二氧化硫对脓毒症大鼠心肌氧化应激的调节[J]. 北京大学学报(医学版), 2023, 55(4): 582-586.
[8] 姬学朝,刘珊,王万州,赵烨彤,李陆一,张文楼,沈国锋,邓芙蓉,郭新彪. 室内挥发性有机物与年轻女性夜间心率变异性关联的定组研究[J]. 北京大学学报(医学版), 2023, 55(3): 488-494.
[9] 哈雪梅,姚永正,孙莉华,辛春杨,熊焰. 实性肺胎盘样变形1例及文献复习[J]. 北京大学学报(医学版), 2023, 55(2): 357-361.
[10] 张宏,董继元,王建军,范临夏,曲强,刘洋. 兰州市臭氧对儿童哮喘的短期影响及其季节性差异[J]. 北京大学学报(医学版), 2022, 54(2): 227-235.
[11] 吴燕芳,高飞,林滇恬,陈志涵,林禾. 托法替布联合治疗抗MDA5抗体阳性的无肌病皮肌炎并发快速进展型间质性肺病5例临床分析[J]. 北京大学学报(医学版), 2021, 53(5): 1012-1016.
[12] 白枫,何倚帆,牛亚楠,杨若娟,曹静. 超细颗粒物对大鼠离体灌注心脏功能的影响[J]. 北京大学学报(医学版), 2021, 53(2): 240-245.
[13] 夏芳芳,鲁芙爱,吕慧敏,杨国安,刘媛. 系统性红斑狼疮伴间质性肺炎的临床特点及相关因素分析[J]. 北京大学学报(医学版), 2021, 53(2): 266-272.
[14] 轩艳,蔡宇,王啸轩,石巧,邱立新,栾庆先. 牙龈卟啉单胞菌感染对载脂蛋白e基因敲除小鼠动脉粥样硬化的影响[J]. 北京大学学报(医学版), 2020, 52(4): 743-749.
[15] 包海荣,刘晓菊,谭恩丽,舒娟,董继元,李盛. 兰州市气温和相对湿度对慢性阻塞性肺疾病患者门诊就诊人次的影响及其交互效应[J]. 北京大学学报(医学版), 2020, 52(2): 308-316.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!