纳米生物活性玻璃促进兔颅骨临界骨缺损修复

doi:10.3969/j.issn.1671-167X.2018.01.007

北京大学学报(医学版) ›› 2018, Vol. 50 ›› Issue (1): 42-48. doi: 10.3969/j.issn.1671-167X.2018.01.007

纳米生物活性玻璃促进兔颅骨临界骨缺损修复

宫玮玉1，刘绍清2，董艳梅1△，高学军1，陈晓峰3

(1. 北京大学口腔医学院·口腔医院，牙体牙髓科口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室，北京100081；2. 北京大学第三医院口腔科，北京100191；3. 华南理工大学生物医学工程中心，广州510641)

出版日期:2018-02-18 发布日期:2018-02-18
通讯作者: 董艳梅 E-mail: kqdongyanmei@bjmu.edu.cn
基金资助:
国家自然科学基金（51372005）和北京大学口腔医院青年科研基金项目(PKUSS20140101)资助

Nano-sized bioactive glass enhances osteogenesis of critical bone defect in rabbits#br#

GONG Wei-yu1, LIU Shao-qing2, DONG Yan-mei1△, GAO Xue-jun1, CHEN Xiao-feng3

(1.Department of Cariology and Endodontology, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, 100081, China; 2. Department of Stomatology, Peking University Third Hospital, Beijing 100191, China; 3. National Engineering Research Center of Human Tissue Restoration & Reconstruction, South China University of Technology, Guangzhou 510641, China)

Online:2018-02-18 Published:2018-02-18
Contact: DONG Yan-mei, E-mail: kqdongyanmei@bjmu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China (51372005) and the Youth Foundation of Peking University School and Hospital of Stomatology (PKUSS20140101)

摘要/Abstract

摘要： 目的：观察并比较58S纳米生物活性玻璃（nano-sized 58S bioactive glass,nano-58S BG）和传统45S5生物活性玻璃（45S5 BG）促进兔顶骨临界骨缺损修复的效果。方法：在新西兰兔顶骨直径9 mm的贯通临界骨缺损中随机填入nano-58S BG或45S5 BG，空白对照组仅以血凝块充盈骨缺损。组织学切片组于术后4周和8周取材制作切片，HE染色和天狼星红苦味酸染色后观察；骨荧光磨片组分别于术后第14、28、42天皮下注射盐酸四环素、茜素红、钙黄绿素标记新生骨，8周取材制成硬组织磨片，激光共聚焦显微镜下观察新骨形成范围，Image J软件定量分析。结果：4周时HE染色可见生物活性玻璃与周围组织紧密结合，未见急慢性炎症细胞浸润，nano-58S组和45S5组可见骨缺损边缘和中央均出现新骨，对照组中央未见新生骨；8周时nano-58S组新生骨多于45S5组和对照组，两个BG组新生骨可见与正常颅骨相同的中空结构，对照组新骨未见中空结构。天狼星红苦味酸染色可见nano-58S组Ⅰ型胶原分泌量大于45S5组和对照组。骨荧光磨片观察显示术后4～6周和6～8周nano58S组新骨形成范围分别为（29.4±4.48）μm和（35.3±3.74）μm，高于45S5组［（13.43±3.44） μm和（17.64±4.13） μm］和对照组［（5.88±2.92） μm和（6.07±3.02） μm，P<0.01］。结论：58S纳米生物活性玻璃促进兔顶骨临界骨缺损修复的效果优于传统的45S5生物活性玻璃。

关键词: 生物活性材料, 生物活性玻璃, 骨再生, 组织工程

Abstract: Objective： To compare the osteogenic effects of a nano-sized 58S bioactive glass (nano-58S BG) and a traditional 45S5 bioactive glass(45S5 BG) in penetrating parietal critical bone defects. Methods: Critical bone defect with 9 mm diameter was created in the parietal bone of New Zealand rabbits. The bone defects were then filled with either nano-58S BG, or 45S5 BG, or nothing but the newly-formed blood clot as the blank control at random. For histological observation, specimens were gained 4 and 8 weeks after the surgery, sectioned and stained by HE. The amount of collagen type Ⅰ was observed with PicricSirius Red staining through polarimetry. To observe the new bone formation with fluorescence under the laser confocal microscope, we injected fluorescent markers 14, 28, and 42 days after the surgery. The markers were tetracycline hydrochloride, alizarin red and calcin individually in chronological order. Image J software was used to quantify the bone regeneration. Results: HE staining showed that BG particulates were integrated with the surrounding tissue without any inflammatory cells infiltration 4 weeks after surgery. New bone regeneration was observed both from the border and in the center of the defects in both BG groups. No bone regeneration in defect center was observed in control group. At the end of 8 weeks, there was more bone regeneration in nano-58S group compared with 45S5 group and control group. The structure of the new bone in BG groups was hollow, which was similar to the natural normal parietal bone. No hollow structure was observed in the new bone of control group. Picric-sirius Red polarimetry showed that more amount of collagen type Ⅰ was found in nano58S group than in either 45S5 or control group. The fluorescent observation of the hard tissue slices at the end of 8 weeks showed statistically larger scope and faster new bone formation in nano-58S group with (29.4±4.48) μm thickness from 4－6 weeks and (35.3±3.74) μm from 6－8 weeks compared with 45S5 group ［(13.43±3.44) μm and (17.64±4.13) μm］ and control group ［(5.88±2.92) μm and (6.07±3.02) μm, P<0.01］. Conclusion: Compared with the traditional 45S5 bioactive glass, 58S nanosized bioactive glass showed better osteogenic effect in bone regeneration in parietal bones of rabbits.

Key words: Bioactive material, Bioactive glass, Bone regeneration, Tissue engineering

中图分类号:

R783.1

宫玮玉，刘绍清，董艳梅，高学军，陈晓峰. 纳米生物活性玻璃促进兔颅骨临界骨缺损修复[J]. 北京大学学报(医学版), 2018, 50(1): 42-48.

GONG Wei-yu, LIU Shao-qing, DONG Yan-mei, GAO Xue-jun, CHEN Xiao-feng. Nano-sized bioactive glass enhances osteogenesis of critical bone defect in rabbits#br#[J]. Journal of Peking University(Health Sciences), 2018, 50(1): 42-48.

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纳米生物活性玻璃促进兔颅骨临界骨缺损修复

Nano-sized bioactive glass enhances osteogenesis of critical bone defect in rabbits#br#

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