Email Alert | RSS

北京大学学报(医学版) ›› 2018, Vol. 50 ›› Issue (3): 527-531. doi: 10.3969/j.issn.1671-167X.2018.03.022

• 论著 • 上一篇    下一篇

唾液基质金属蛋白酶2、9与儿童龋病相关性的初步研究

王潇，王欣，秦满△   

  1. （北京大学口腔医学院·口腔医院，儿童口腔科国家口腔疾病临床医学研究中心口腔数字化医疗技术和材料国家工程实验室口腔数字医学北京市重点实验室， 北京100081）
  • 出版日期:2018-06-18 发布日期:2018-06-18
  • 通讯作者: 秦满 E-mail:qin-man@foxmail.com
  • 基金资助:
    北京大学口腔医学院院内博士后种子基金（YS0203）资助

A preliminary study of saliva matrix metalloproteinases (MMP-2 and MMP-9) in children with caries

WANG Xiao, WANG Xin, QIN Man△   

  1. (Department of Pediatric Dentistry, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China)
  • Online:2018-06-18 Published:2018-06-18
  • Contact: QIN Man E-mail:qin-man@foxmail.com
  • Supported by:
    Supported by the Postdoctoral Seed Foundation of Peking University School and Hospital of Stomatology

RICH HTML

  

PDF (PC)

摘要： 目的：比较健康儿童和不同龋损程度儿童唾液中基质金属蛋白酶（matrix metalloproteinase，MMP）-2、MMP-9含量差异以及重度龋儿童治疗前后唾液中MMP2、MMP-9的蛋白水平变化，探讨其与儿童龋病发生、发展的相关性。方法：纳入368名3~5岁儿童，根据牙齿龋坏程度分为重度龋组112人、轻度龋组98人和无龋组158人，重度龋组患儿中完成全面的龋齿治疗并随访取样的83人纳入治疗组，测定唾液中MMP-2，MMP-9水平。使用SPSS 13.0软件分析数据，重度龋组、轻度龋组与无龋组之间的比较采用SNK-q法，重度龋组与治疗组之间的比较采用配对t检验。结果：重度龋组、轻度龋组、无龋组和治疗组的年龄及性别构成差异无统计学意义。重度龋组唾液中的MMP-2含量［(141.3±32.5) μg/L］高于轻度龋组［(107.5±21.3)μg/L］和无龋组［(102.8±18.5)μg/L］（P均＜0.05），轻度龋组与无龋组间差异无统计学意义（P＞0.05）。对纳入治疗组的83人进行分析，唾液中MMP2含量［(120.1±24.8)μg/L］低于治疗前［(144.6±30.3)μg/L］（P＜0.05），但仍高于无龋组（P＜0.05）。重度龋组［(445.8±68.1)μg/L］和轻度龋组［(428.6±59.2)μg/L］唾液MMP9含量高于无龋组［(385.4±60.6)μg/L］（P均＜0.05），重度龋组与轻度龋组间差异无统计学意义（P＞0.05）。对纳入治疗组的83人进行分析，唾液中MMP9含量［(432.2±64.7)μg/L］与治疗前［(440.1±75.5)μg/L］没有明显变化（P＞0.05）。结论：重度龋患儿唾液中MMP-2、MMP-9含量显著高于无龋儿童，即使经过治疗仍高于健康儿童，提示唾液中的MMP-2、MMP-9可能是儿童患龋的相关影响因素。

关键词:  , 龋齿, 基质金属蛋白酶2, 基质金属蛋白酶9, 唾液, 儿童

Abstract: Objective： To investigate the correlation between matrix metalloproteinase (MMP)-2/MMP-9 levels and childhood caries, and the saliva levels of MMP-2/MMP-9 among healthy children and those with different degrees of dental caries, both before and after treatment. Methods: In the study, 368 children aged 3 to 5 years were separated into three groups: severe caries group (112 children), mild caries group (98 children) and caries free group (158 children). The children with severe caries were included in treatment group (83 children) after accepting a comprehensive treatment of caries. MMP-2 and MMP-9 levels were determined by enzyme-linked immunosorbent assay (ELISA) and the data were analyzed by the Statistics Package for Social Science (SPSS 13.0). The differences among severe caries group, mild caries group and caries free group were analyzed by SNK-q (Student Newman Keuls). The severe caries group and treatment group were compared by paired t test. The differences between each group were statistically analyzed. Results: There was no significant difference of the age and gender composition among severe caries group, mild caries group, caries free group and treatment group. The MMP-2 level of severe caries group ［(141.3±32.5) μg/L］ was higher than those of mild caries group ［(107.5±21.3) μg/L］ and caries free group ［(102.8±18.5) μg/L］ (P<0.05). There was no significant difference between mild caries and caries free group (P>0.05). After analysis of 83 children in the treatment group, the level of MMP2 ［(120.1±24.8) μg/L］ was lower than before ［(144.6±30.3) μg/L］ (P<0.05), but was higher than that of caries free group (P<0.05). The MMP-9 levels of severe caries group ［(445.8±68.1) μg/L］ and mild caries group ［(428.6±59.2) μg/L］ were higher than that of caries free group ［(385.4±60.6) μg/L］ (P<0.05), but the difference between severe caries group and mild caries group was not significant (P>0.05). After analysis of 83 children in the treatment group, the alteration of MMP-9 ［(432.2±64.7) μg/L］ was not significant either (P>0.05). Conclusion: The saliva levels of MMP-2 and MMP-9 in children with severe caries were higher than those in caries free children, even if the treatment was implemented, which suggests that the MMP-2 and MMP-9 in saliva might be related to the caries in children.

Key words: Dental caries, Matrix metalloproteinase 2, Matrix metalloproteinase 9, Saliva, Child

中图分类号: 

  • R788.1
[1] 赵双云, 邹思雨, 李雪莹, 沈丽娟, 周虹. 中文版口腔健康素养量表简版(HeLD-14)在学龄前儿童家长中应用的信度和效度评价[J]. 北京大学学报（医学版）, 2024, 56(5): 828-832.
[2] 陈心心, 唐哲, 乔艳春, 荣文笙. 北京市密云区4岁儿童患龋状况及其与龋活跃性检测的相关性[J]. 北京大学学报（医学版）, 2024, 56(5): 833-838.
[3] 杨玉淑, 齐晅, 丁萌, 王炜, 郭惠芳, 高丽霞. 抗唾液腺蛋白1抗体联合抗腮腺分泌蛋白抗体对干燥综合征的诊断价值[J]. 北京大学学报（医学版）, 2024, 56(5): 845-852.
[4] 岳芷涵,韩娜,鲍筝,吕瑾莨,周天一,计岳龙,王辉,刘珏,王海俊. 儿童早期体重指数轨迹与超重风险关联的前瞻性队列研究[J]. 北京大学学报（医学版）, 2024, 56(3): 390-396.
[5] 费秀文,刘斯,汪波,董爱梅. 成人及儿童组织坏死性淋巴结炎临床特征及治疗[J]. 北京大学学报（医学版）, 2024, 56(3): 533-540.
[6] 俞光岩. 儿童唾液腺疾病[J]. 北京大学学报（医学版）, 2024, 56(1): 1-3.
[7] 闫晓晋,刘云飞,马宁,党佳佳,张京舒,钟盼亮,胡佩瑾,宋逸,马军. 《中国儿童发展纲要(2011-2020年)》实施期间中小学生营养不良率变化及其政策效应分析[J]. 北京大学学报（医学版）, 2023, 55(4): 593-599.
[8] 弭小艺,侯杉杉,付子苑,周末,李昕璇,孟召学,蒋华芳,周虹. 中文版童年不良经历问卷在学龄前儿童父母中应用的信效度评价[J]. 北京大学学报（医学版）, 2023, 55(3): 408-414.
[9] 崔孟杰,马奇,陈曼曼,马涛,王鑫鑫,刘婕妤,张奕,陈力,蒋家诺,袁雯,郭桐君,董彦会,马军,星一. 不同生长模式与7~17岁儿童青少年代谢综合征的关系[J]. 北京大学学报（医学版）, 2023, 55(3): 415-420.
[10] 党佳佳,蔡珊,钟盼亮,王雅琪,刘云飞,师嫡,陈子玥,张依航,胡佩瑾,李晶,马军,宋逸. 室外夜间人工光暴露与中国9~18岁儿童青少年超重肥胖的关联[J]. 北京大学学报（医学版）, 2023, 55(3): 421-428.
[11] 陈敬,肖伍才,单蕊,宋洁云,刘峥. DRD2基因rs2587552多态性对儿童肥胖干预效果的影响：一项前瞻性、平行对照试验[J]. 北京大学学报（医学版）, 2023, 55(3): 436-441.
[12] 俞光岩,宿骞,张艳,吴立玲. 唾液腺疾病与全身系统性疾病的相关性[J]. 北京大学学报（医学版）, 2023, 55(1): 1-7.
[13] 柳登高,郑丹妮,赵雅宁,张亚琼,叶欣,张丽琪,谢晓艳,张雷,张祖燕,俞光岩. 疑难唾液腺结石病的治疗研究[J]. 北京大学学报（医学版）, 2023, 55(1): 8-12.
[14] 刘杨,程昉,王艳玲,艾香艳,朱振航,赵福涛. 唾液腺超声对干燥综合征的诊断价值[J]. 北京大学学报（医学版）, 2022, 54(6): 1123-1127.
[15] 李辉,高阳旭,王书磊,姚红新. 恶性肿瘤患儿完全植入式静脉输液港手术并发症[J]. 北京大学学报（医学版）, 2022, 54(6): 1167-1171.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
ISSN 1671-167X
CN 11-4691/R
主管单位：中华人民共和国教育部
主办单位：北京大学
地址： 北京市海淀区学院路38号 北京大学医学部 《北京大学学报（医学版）》
邮编：100191
电话：010-82801551
Email： xbbjb2@bjmu.edu.cn

《北京大学学报（医学版）》
微信公众号
版权所有 © 2018 《北京大学学报（医学版）》编辑部